Autor según el artículo: Montori-Grau M; Pedreira-Casahuga R; Boyer-Díaz Z; Lassot I; García-Martínez C; Orozco A; Cebrià J; Osorio-Conles O; Chacón M; Vendrell J; Vázquez-Carrera M; Desagher S; Jiménez-Chillarón J; Gómez-Foix A
Departamento: Medicina i Cirurgia
Autor/es de la URV: Vendrell Ortega, Juan José
Palabras clave: Trim7: trim7 isoform 4 Synthase kinase-3 Skeletal muscle Protein Gnip1: trim7 isoform 1 Glycogen metabolism Glucose-uptake Adenovirus-mediated transfer Activation skeletal muscle gnip1: trim7 isoform 1 glycogen metabolism
Resumen: Glycogenin-interacting protein 1 (GNIP1) is a tripartite motif (TRIM) protein with E3 ubiquitin ligase activity that interacts with glycogenin. These data suggest that GNIP1 could play a major role in the control of glycogen metabolism. However, direct evidence based on functional analysis remains to be obtained.The aim of this study was 1) to define the expression pattern of glycogenin-interacting protein/Tripartite motif containing protein 7 (GNIP/TRIM7) isoforms in humans, 2) to test their ubiquitin E3 ligase activity, and 3) to analyze the functional effects of GNIP1 on muscle glucose/glycogen metabolism both in human cultured cells and in vivo in mice.We show that GNIP1 was the most abundant GNIP/TRIM7 isoform in human skeletal muscle, whereas in cardiac muscle only TRIM7 was expressed. GNIP1 and TRIM7 had autoubiquitination activity in vitro and were localized in the Golgi apparatus and cytosol respectively in LHCN-M2 myoblasts. GNIP1 overexpression increased glucose uptake in LHCN-M2 myotubes. Overexpression of GNIP1 in mouse muscle in vivo increased glycogen content, glycogen synthase (GS) activity and phospho-GSK-3?/? (Ser21/9) and phospho-Akt (Ser473) content, whereas decreased GS phosphorylation in Ser640. These modifications led to decreased blood glucose levels, lactate levels and body weight, without changing whole-body insulin or glucose tolerance in mouse.GNIP1 is an ubiquitin ligase with a markedly glycogenic effect in skeletal muscle.Copyright © 2018 Elsevier Inc. All rights reserved.
Áreas temáticas: Saúde coletiva Odontología Nutrição Medicina iii Medicina ii Medicina i Interdisciplinar General medicine Farmacia Engenharias ii Enfermagem Endocrinology, diabetes and metabolism Endocrinology & metabolism Endocrinology Educação física Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Ciência de alimentos Biotecnología Antropologia / arqueologia
Acceso a la licencia de uso: https://creativecommons.org/licenses/by/3.0/es/
ISSN: 00260495
Direcció de correo del autor: juanjose.vendrell@urv.cat
Identificador del autor: 0000-0002-6994-6115
Fecha de alta del registro: 2024-09-07
Versión del articulo depositado: info:eu-repo/semantics/acceptedVersion
Enlace a la fuente original: https://www.metabolismjournal.com/article/S0026-0495(18)30044-1/fulltext
URL Documento de licencia: https://repositori.urv.cat/ca/proteccio-de-dades/
Referencia al articulo segun fuente origial: Metabolism-Clinical And Experimental. 83 177-187
Referencia de l'ítem segons les normes APA: Montori-Grau M; Pedreira-Casahuga R; Boyer-Díaz Z; Lassot I; García-Martínez C; Orozco A; Cebrià J; Osorio-Conles O; Chacón M; Vendrell J; Vázquez-Car (2018). GNIP1 E3 ubiquitin ligase is a novel player in regulating glycogen metabolism in skeletal muscle. Metabolism-Clinical And Experimental, 83(), 177-187. DOI: 10.1016/j.metabol.2018.02.005
DOI del artículo: 10.1016/j.metabol.2018.02.005
Entidad: Universitat Rovira i Virgili
Año de publicación de la revista: 2018
Tipo de publicación: Journal Publications
Repositori URV