Autor según el artículo: Ortiz, Mayreli; Fragoso, Alex; O'Sullivan, Ciara K
Departamento: Enginyeria Química
Autor/es de la URV: Fragoso Sierra, Alex / O'SULLIVAN, CIARA KATHLEEN / Ortíz Rodríguez, Mayreli
Palabras clave: Autoantibodies; Beta-cyclodextrins; Betadex; Biosensing techniques; Celiac disease; Enzyme-linked immunosorbent assay; Gliadin; Gold; Humans; Macromolecular substances; Molecular conformation; Monolayers; Proteins; Surface properties
Resumen: Celiac disease is a condition associated with the ingestion of gluten by genetically susceptible individuals. Measurement of serum antigliadin antibodies is a diagnostic tool also used as a means of monitoring a patient's compliance to a gluten-free diet. In this work, we demonstrate the applicability of an electrochemical supramolecular platform based on cyclodextrin-modified gold surfaces to detect antigliadin antibodies in real serum samples. Several support layer-biorecognition element combinations were tested in order to maximize the electrochemical response, and the assay was optimized in terms of incubation times and resistance to nonspecific interactions. The developed supramolecular biosensor was then applied to the amperometric detection of antigliadin IgA and IgG autoantibodies in real samples of celiac disease patients under follow-up treatment; the results were compared with a commercial enzyme linked immunosorbent assay (ELISA) test, and an excellent correlation was observed between both methods.© 2011 American Chemical Society
Áreas temáticas: Analytical chemistry; Astronomia / física; Biodiversidade; Biotecnología; Chemistry, analytical; Ciência da computação; Ciência de alimentos; Ciências agrárias i; Ciências ambientais; Ciências biológicas i; Ciências biológicas ii; Ciências biológicas iii; Enfermagem; Engenharias ii; Engenharias iii; Engenharias iv; Ensino; Farmacia; General medicine; Geociências; Interdisciplinar; Materiais; Medicina i; Medicina ii; Química
Acceso a la licencia de uso: https://creativecommons.org/licenses/by/3.0/es/
Direcció de correo del autor: alex.fragoso@urv.cat; mayreli.ortiz@urv.cat; mayreli.ortiz@urv.cat
Fecha de alta del registro: 2025-03-15
Versión del articulo depositado: info:eu-repo/semantics/acceptedVersion
Enlace a la fuente original: https://pubs.acs.org/doi/10.1021/ac102956p
Referencia al articulo segun fuente origial: Analytical Chemistry. 83 (8): 2931-2938
Referencia de l'ítem segons les normes APA: Ortiz, Mayreli; Fragoso, Alex; O'Sullivan, Ciara K (2011). Detection of antigliadin autoantibodies in celiac patient samples using a cyclodextrin-based supramolecular biosensor. Analytical Chemistry, 83(8), 2931-2938. DOI: 10.1021/ac102956p
URL Documento de licencia: https://repositori.urv.cat/ca/proteccio-de-dades/
DOI del artículo: 10.1021/ac102956p
Entidad: Universitat Rovira i Virgili
Año de publicación de la revista: 2011
Tipo de publicación: Journal Publications
Repositori URV