Autor según el artículo: Simó A; Just-Borràs L; Cilleros-Mañé V; Hurtado E; Nadal L; Tomàs M; Garcia N; Lanuza M; Tomàs J

Departamento: Ciències Mèdiques Bàsiques

Autor/es de la URV: Cilleros Mañé, Víctor / Garcia Sancho, Maria de les Neus / Just Borràs, Laia / Lanuza Escolano, María Angel / NADAL MAGRIÑÀ, LAURA / SIMÓ OLLÉ, ANNA / Tomás Ferré, José Maria / Tomas Marginet, Marta

Palabras clave: Synaptic vesicles Pkc isoforms Pkc Nmj Neurotrophic factors Neurotransmission Neuromuscular junction Muscle contraction Munc18-1 Bdnf-trkb signaling Bdnf-trkb pathway pkc isoforms neurotrophic factors neurotransmission neuromuscular junction muscle contraction munc18-1 bdnf-trkb pathway

Resumen: © 2018 Simó, Just-Borràs, Cilleros-Mañé, Hurtado, Nadal, Tomàs, Garcia, Lanuza and Tomàs. Munc18-1, a neuron-specific member of the Sec1/Munc18 family, is involved in neurotransmitter release by binding tightly to syntaxin. Munc18-1 is phosphorylated by PKCon Ser-306 andSer-313 in vitro which reduces the amount of Munc18-1 able to bind syntaxin. We have previously identified that PKC is involved in neurotransmitter release when continuous electrical stimulation imposes a moderate activity on the NMJ and that muscle contraction through TrkB has an important impact on presynaptic PKC isoforms levels, specifically cPKCβI and nPKCε. Therefore, the present study was designed to understand how Munc18-1 phosphorylation is affected by (1) synaptic activity at the neuromuscular junction, (2) nPKCε and cPKCβI isoforms activity, (3) muscle contraction per se, and (4) the BDNF/TrkB signaling in a neuromuscular activity-dependent manner. We performed immunohistochemistry and confocal techniques to evidence the presynaptic location of Munc18-1 in the rat diaphragmmuscle. To study synaptic activity, we stimulated the phrenic nerve (1Hz, 30min) with or without contraction (abolished by µ-conotoxin GIIIB). Specific inhibitory reagents were used to block nPKCε and cPKCβI activity and to modulate the tropomyosin receptor kinase B (TrkB). Main results obtained from Western blot experiments showed that phosphorylation of Munc18-1 at Ser-313 increases in response to a signaling mechanism initiated by synaptic activity and directly mediated by nPKCε. Otherwise, cPKCβI and TrkB activities work together to prevent this synaptic activity–induced Munc18-1 phosphorylation by a negative regulation of cPKCβI over nPKCε. Therefore, a balance between the activities of these PKC isoforms could be a relevant cue in the regulation of the exocytotic apparatus. The results also demonstrate that muscle contraction prevents the synaptic activity–induced Munc18-1 phosphorylation through a mechanism that opposes the TrkB/cPKCβI/nPKCε signaling.

Áreas temáticas: Neurosciences Molecular biology Medicina ii Ciências biológicas ii Cellular and molecular neuroscience

Acceso a la licencia de uso: https://creativecommons.org/licenses/by/3.0/es/

ISSN: 16625099

Direcció de correo del autor: victor.cilleros@urv.cat marta.tomas@urv.cat mariaangel.lanuza@urv.cat laia.just@urv.cat josepmaria.tomas@urv.cat victor.cilleros@urv.cat

Identificador del autor: 0000-0001-5690-9932 0000-0002-4151-1697 0000-0003-4795-4103 0000-0003-0473-3730 0000-0002-0406-0006 0000-0001-5690-9932

Fecha de alta del registro: 2023-09-23

Volumen de revista: 11

Versión del articulo depositado: info:eu-repo/semantics/publishedVersion

Enlace a la fuente original: https://www.frontiersin.org/articles/10.3389/fnmol.2018.00207/full

URL Documento de licencia: http://repositori.urv.cat/ca/proteccio-de-dades/

Referencia al articulo segun fuente origial: Frontiers In Molecular Neuroscience. 11 207-

Referencia de l'ítem segons les normes APA: Simó A; Just-Borràs L; Cilleros-Mañé V; Hurtado E; Nadal L; Tomàs M; Garcia N; Lanuza M; Tomàs J (2018). BDNF-TrKB signaling coupled to nPKCε and cPKCβI modulate the phosphorylation of the exocytotic protein MUNC18-1 during synaptic activity at the neuromuscular junction. Frontiers In Molecular Neuroscience, 11(), 207-. DOI: 10.3389/fnmol.2018.00207

DOI del artículo: 10.3389/fnmol.2018.00207

Entidad: Universitat Rovira i Virgili

Año de publicación de la revista: 2018

Página inicial: 207

Tipo de publicación: Journal Publications