Articles producció científica> Enginyeria Química

Monoclonal antibodies to high molecula weight glutenin subunits for use in measurement of gluten in foods

  • Datos identificativos

    Identificador: imarina:5129533
    Handle: http://hdl.handle.net/20.500.11797/imarina5129533
  • Autores:

    H Julia Ellis
    M Carmen Bermudo Redondo
    Tanja Suligoj
    Nika Japelij
    Nuria Gonzalez-Cinca
    Beatriz Côrte-Real
    Paul J Ciclitira
  • Otros:

    Autor según el artículo: H Julia Ellis; M Carmen Bermudo Redondo; Tanja Suligoj; Nika Japelij; Nuria Gonzalez-Cinca; Beatriz Côrte-Real; Paul J Ciclitira
    Departamento: Enginyeria Química
    Autor/es de la URV: Bermudo Redondo, Maria del Carmen
    Resumen: Background: Celiac disease (CD) is a heritable intolerance to gluten, the s t o r a g e protein found in wheat, rye, and barley. Commercially available assays used to calculate gluten content in food do not measure CD toxic high molecular weight glutenins (HMWG), but rely on the calculation that gliadin multiplied by two equals total gluten, which is invalid. Objective: We sought to generate monoclonal antibodies (mAbs), to known CD toxic HMWG subunits (HMW-GS), to develop improved assays to quantify CD toxic gluten in foods. Design: mAbs were generated using recombinant HMW-GS as antigens. They were termed CDC-3 and CDC-7 and were characterised by dot blot assay and ELISA. A suitable mAb was used in competitive ELISA to quantify HMW-GS content in foods intended to CD suffers. Results: Antibodies were raised against the important dough forming HMW-GS, 1Dy10 that cross-reacted with another important dough forming HMW-GS, 1Dx5. mAbs cross-reacted at high titre with HMW-GS extracted from fifteen varieties of European bread wheat. Competitive ELISA had a limit of detection of 625pg/ml, which is equivalent to 6.25x10 -3 ppm in a food extract. Conclusion: Our new antibodies react with a wide variety of wheat cultivars and can be used in sensitive ELISA assays for detection and quantification of CD toxic HMW-GS in foods intended to CD suffers. Summation of the food content of HMW-GS and gliadin permits improved quantitation of CD toxic gluten in nominally gluten-free foods.
    Áreas temáticas: Saúde coletiva Nutrição Educação física
    Acceso a la licencia de uso: https://creativecommons.org/licenses/by/3.0/es/
    Direcció de correo del autor: carmen.bermudo@urv.cat
    Identificador del autor: 0000-0002-4128-9803
    Fecha de alta del registro: 2023-11-27
    Versión del articulo depositado: info:eu-repo/semantics/publishedVersion
    Enlace a la fuente original: http://verizonaonlinepublishing.com/CurrentIssueFoodTwo.aspx
    Referencia al articulo segun fuente origial: Food And Nutrition Report. 1 (2): 10-18
    Referencia de l'ítem segons les normes APA: H Julia Ellis; M Carmen Bermudo Redondo; Tanja Suligoj; Nika Japelij; Nuria Gonzalez-Cinca; Beatriz Côrte-Real; Paul J Ciclitira (2015). Monoclonal antibodies to high molecula weight glutenin subunits for use in measurement of gluten in foods. Food And Nutrition Report, 1(2), 10-18. DOI: 10.24218/fnr.2015.08
    URL Documento de licencia: http://repositori.urv.cat/ca/proteccio-de-dades/
    DOI del artículo: 10.24218/fnr.2015.08
    Entidad: Universitat Rovira i Virgili
    Año de publicación de la revista: 2015
    Tipo de publicación: info:eu-repo/semantics/article
  • Palabras clave:

    Celiac disease, Gluten, Wheat, High molecular weight glutenins, ELISA
    Saúde coletiva
    Nutrição
    Educação física
  • Documentos:

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