Articles producció científica> Medicina i Cirurgia

Collagen and fibronectin surface modification of nanoporous anodic alumina and macroporous silicon for endothelial cell cultures

  • Datos identificativos

    Identificador: imarina:5132387
    Autores:
    Formentin, PCatalan, UPol, LFernandez-Castillejo, SSola, RMarsal, L F
    Resumen:
    Background The ability to direct the cellular response by means of biomaterial surface topography is important for biomedical applications. Substrate surface topography has been shown to be an effective cue for the regulation of cellular response. Here, the response of human aortic endothelial cells to nanoporous anodic alumina and macroporous silicon with collagen and fibronectin functionalization has been studied. Methods Confocal microscopy and scanning electron microscopy were employed to analyse the effects of the material and the porosity on the adhesion, morphology, and proliferation of the cells. Cell spreading and filopodia formation on macro- and nanoporous material was characterized by atomic force microscopy. We have also studied the influence of the protein on the adhesion. Results It was obtained the best results when the material is functionalized with fibronectin, regarding cells adhesion, morphology, and proliferation. Conclusion These results permit to obtain chemical modified 3D structures for several biotechnology applications such as tissue engineering, organ-on-chip or regenerative medicine.
  • Otros:

    Autor según el artículo: Formentin, P; Catalan, U; Pol, L; Fernandez-Castillejo, S; Sola, R; Marsal, L F
    Departamento: Medicina i Cirurgia Enginyeria Electrònica, Elèctrica i Automàtica
    Autor/es de la URV: Catalán Santos, Úrsula / FERNÁNDEZ CASTILLEJO, SARA / Formentín Vallés, Pilar / Marsal Garví, Luis Francisco / Solà Alberich, Rosa Maria
    Palabras clave: Surface properties Nanoporous anodic alumina Macroporous silicon Fibronectin Endothelial cells Collagen adhesion, morphology and proliferation nanoporous anodic alumina macroporous silicon fibronectin endothelial cells collagen adhesion, morphology and proliferation
    Resumen: Background The ability to direct the cellular response by means of biomaterial surface topography is important for biomedical applications. Substrate surface topography has been shown to be an effective cue for the regulation of cellular response. Here, the response of human aortic endothelial cells to nanoporous anodic alumina and macroporous silicon with collagen and fibronectin functionalization has been studied. Methods Confocal microscopy and scanning electron microscopy were employed to analyse the effects of the material and the porosity on the adhesion, morphology, and proliferation of the cells. Cell spreading and filopodia formation on macro- and nanoporous material was characterized by atomic force microscopy. We have also studied the influence of the protein on the adhesion. Results It was obtained the best results when the material is functionalized with fibronectin, regarding cells adhesion, morphology, and proliferation. Conclusion These results permit to obtain chemical modified 3D structures for several biotechnology applications such as tissue engineering, organ-on-chip or regenerative medicine.
    Áreas temáticas: Molecular biology Medicina i Environmental engineering Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Cell biology Biotechnology & applied microbiology Biomedical engineering Biochemical research methods
    Acceso a la licencia de uso: https://creativecommons.org/licenses/by/3.0/es/
    ISSN: 17541611
    Direcció de correo del autor: ursula.catalan@urv.cat rosa.sola@urv.cat lluis.marsal@urv.cat pilar.formentin@urv.cat pilar.formentin@urv.cat
    Identificador del autor: 0000-0001-8884-9823 0000-0002-8359-235X 0000-0002-5976-1408
    Fecha de alta del registro: 2024-10-12
    Versión del articulo depositado: info:eu-repo/semantics/publishedVersion
    Enlace a la fuente original: https://jbioleng.biomedcentral.com/articles/10.1186/s13036-018-0111-x
    URL Documento de licencia: https://repositori.urv.cat/ca/proteccio-de-dades/
    Referencia al articulo segun fuente origial: Journal Of Biological Engineering. 12 (21): 21-
    Referencia de l'ítem segons les normes APA: Formentin, P; Catalan, U; Pol, L; Fernandez-Castillejo, S; Sola, R; Marsal, L F (2018). Collagen and fibronectin surface modification of nanoporous anodic alumina and macroporous silicon for endothelial cell cultures. Journal Of Biological Engineering, 12(21), 21-. DOI: 10.1186/s13036-018-0111-x
    DOI del artículo: 10.1186/s13036-018-0111-x
    Entidad: Universitat Rovira i Virgili
    Año de publicación de la revista: 2018
    Tipo de publicación: Journal Publications
  • Palabras clave:

    Biochemical Research Methods,Biomedical Engineering,Biotechnology & Applied Microbiology,Cell Biology,Environmental Engineering,Molecular Biology
    Surface properties
    Nanoporous anodic alumina
    Macroporous silicon
    Fibronectin
    Endothelial cells
    Collagen adhesion, morphology and proliferation
    nanoporous anodic alumina
    macroporous silicon
    fibronectin
    endothelial cells
    collagen adhesion, morphology and proliferation
    Molecular biology
    Medicina i
    Environmental engineering
    Ciências biológicas iii
    Ciências biológicas ii
    Ciências biológicas i
    Cell biology
    Biotechnology & applied microbiology
    Biomedical engineering
    Biochemical research methods
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