Autor según el artículo: Fernández-Bravo A, López-Fernández L, Figueras MJ

Departamento: Ciències Mèdiques Bàsiques

Autor/es de la URV: Fernández Bravo, Ana / Figueras Salvat, María Josefa

Palabras clave: Ureases Urease maturation System Site Ros Resistance Nife hydrogenase Metals Metallochaperone Mechanism Macrophages Hydrogenases Hsp33 Escherichia-coli Chaperone Alkaline Aeromonas Acid

Resumen: Metallochaperones are essential proteins that insert metal ions or metal cofactors into specific enzymes, that after maturation will become metalloenzymes. One of the most studied metallochaperones is the nickel-binding protein HypA, involved in the maturation of nickel-dependent hydrogenases and ureases. HypA was previously described in the human pathogens Escherichia coli and Helicobacter pylori and was considered a key virulence factor in the latter. However, nothing is known about this metallochaperone in the species of the emerging pathogen genus Aeromonas. These bacteria are native inhabitants of aquatic environments, often associated with cases of diarrhea and wound infections. In this study, we performed an in silico study of the hypA gene on 36 Aeromonas species genomes, which showed the presence of the gene in 69.4% (25/36) of the Aeromonas genomes. The similarity of Aeromonas HypA proteins with the H. pylori orthologous protein ranged from 21-23%, while with that of E. coli it was 41-45%. However, despite this low percentage, Aeromonas HypA displays the conserved characteristic metal-binding domains found in the other pathogens. The transcriptional analysis enabled the determination of hypA expression levels under acidic and alkaline conditions and after macrophage phagocytosis. The transcriptional regulation of hypA was found to be pH-dependent, showing upregulation at acidic pH. A higher upregulation occurred after macrophage infection. This is the first study that provided evidence that the HypA metallochaperone in Aeromonas might play a role in acid tolerance and in the defense against macrophages.

Áreas temáticas: Virology Microbiology (medical) Microbiology

Acceso a la licencia de uso: https://creativecommons.org/licenses/by/3.0/es/

ISSN: 20762607

Direcció de correo del autor: ana.fernandez@urv.cat mariajose.figueras@urv.cat

Identificador del autor: 0000-0001-7637-5958 0000-0002-2268-8980

Fecha de alta del registro: 2023-03-05

Versión del articulo depositado: info:eu-repo/semantics/publishedVersion

Enlace a la fuente original: https://www.mdpi.com/2076-2607/7/10/415

URL Documento de licencia: http://repositori.urv.cat/ca/proteccio-de-dades/

Referencia al articulo segun fuente origial: Microorganisms. 7 (10):

Referencia de l'ítem segons les normes APA: Fernández-Bravo A, López-Fernández L, Figueras MJ (2019). The Metallochaperone Encoding Gene hypA Is Widely Distributed among Pathogenic Aeromonas spp. Microorganisms, 7(10), -. DOI: 10.3390/microorganisms7100415

DOI del artículo: 10.3390/microorganisms7100415

Entidad: Universitat Rovira i Virgili

Año de publicación de la revista: 2019

Tipo de publicación: Journal Publications