Autor según el artículo: Rodríguez-Luna A; Ávila-Román J; Oliveira H; Motilva V; Talero E

Departamento: Bioquímica i Biotecnologia

Autor/es de la URV: Ávila Román, Francisco Javier

Palabras clave: Vulgaris Uvb Skin Rosmarinic acid Photo-protection Pathway Oxidative stress Nrlp3 Inhibition Inflammasome Growth Fucoxanthin Expression Dna-damage Cells Anti-oxidative Anti-inflammatory Activation uvb photo-protection nrlp3 inflammasome fucoxanthin anti-oxidative anti-inflammatory

Resumen: © 2019 by the authors. Excessive exposure to ultraviolet (UV) radiation is the main risk factor to develop skin pathologies or cancer because it encourages oxidative condition and skin inflammation. In this sense, strategies for its prevention are currently being evaluated. Natural products such as carotenoids or polyphenols, which are abundant in the marine environment, have been used in the prevention of oxidative stress due to their demonstrated antioxidant activities. Nevertheless, the anti-inflammatory activity and its implication in photo-prevention have not been extensively studied. Thus, we aimed to evaluate the combination of fucoxanthin (FX) and rosmarinic acid (RA) on cell viability, apoptosis induction, inflammasome regulation, and anti-oxidative response activation in UVB-irradiated HaCaT keratinocytes. We demonstrated for the first time that the combination of FX and RA (5 µM RA plus 5 µM FX, designated as M2) improved antioxidant and anti-inflammatory profiles in comparison to compounds assayed individually, by reducing UVB-induced apoptosis and the consequent ROS production. Furthermore, the M2 combination modulated the inflammatory response through down-regulation of inflammasome components such as NLRP3, ASC, and Caspase-1, and the interleukin (IL)-1β production. In addition, Nrf2 and HO-1 antioxidant genes expression increased in UVB-exposed HaCaT cells pre-treated with M2. These results suggest that this combination of natural products exerts photo-protective effects by down-regulating NRLP3-inflammasome and increasing Nrf2 signalling pathway.

Áreas temáticas: Zootecnia / recursos pesqueiros Química Pharmacology, toxicology and pharmaceutics (miscellaneous) Pharmacology & pharmacy Pharmaceutical science Nutrição Medicina veterinaria Medicina iii Medicina ii Medicina i Interdisciplinar Geociências Farmacia Engenharias iii Engenharias ii Educação física Drug discovery Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Ciências ambientais Ciências agrárias i Ciência de alimentos Chemistry, medicinal Biotecnología Biodiversidade Astronomia / física

Acceso a la licencia de uso: https://creativecommons.org/licenses/by/3.0/es/

ISSN: 16603397

Direcció de correo del autor: franciscojavier.avila@urv.cat

Identificador del autor: 0000-0001-9766-8178

Fecha de alta del registro: 2023-02-18

Versión del articulo depositado: info:eu-repo/semantics/publishedVersion

Enlace a la fuente original: https://www.mdpi.com/1660-3397/17/8/451

URL Documento de licencia: http://repositori.urv.cat/ca/proteccio-de-dades/

Referencia al articulo segun fuente origial: Marine Drugs. 17 (8):

Referencia de l'ítem segons les normes APA: Rodríguez-Luna A; Ávila-Román J; Oliveira H; Motilva V; Talero E (2019). Fucoxanthin and rosmarinic acid combination has anti-inflammatory effects through regulation of NLRP3 inflammasome in UVB-exposed HaCaT keratinocytes. Marine Drugs, 17(8), -. DOI: 10.3390/md17080451

DOI del artículo: 10.3390/md17080451

Entidad: Universitat Rovira i Virgili

Año de publicación de la revista: 2019

Tipo de publicación: Journal Publications