Articles producció científica> Ciències Mèdiques Bàsiques

The novel protein kinase C epsilon isoform modulates acetylcholine release in the rat neuromuscular junction

  • Datos identificativos

    Identificador: imarina:6388525
    Handle: http://hdl.handle.net/20.500.11797/imarina6388525
  • Autores:

    Obis T
    Hurtado E
    Nadal L
    Tomàs M
    Priego M
    Simon A
    Garcia N
    Santafe M
    Lanuza M
    Tomàs J
  • Otros:

    Autor según el artículo: Obis T; Hurtado E; Nadal L; Tomàs M; Priego M; Simon A; Garcia N; Santafe M; Lanuza M; Tomàs J
    Departamento: Ciències Mèdiques Bàsiques
    Autor/es de la URV: Garcia Sancho, Maria de les Neus / HURTADO CABALLERO, ERICA / Lanuza Escolano, María Angel / NADAL MAGRIÑÀ, LAURA / Santafé Martínez, Manuel / SIMÓ OLLÉ, ANNA / Tomás Ferré, José Maria / Tomas Marginet, Marta
    Palabras clave: Transmitter release Skeletal-muscle Signal-transduction pathway Protein kinase c Protein kinase a Presynaptic muscarinic autoreceptors Pkc-epsilon Pkc epsilon Pkc Neurotransmitter release Neurotransmission Neuromuscular junction Muscarinic receptors Motor nerve terminal Induced cell-death Electrical stimulation Ca2+ Ca 2+ Anchoring proteins Adult-rat Adenosine receptors Ach release Acetylcholine release
    Resumen: © 2015 Obis et al. Background: Various protein kinase C (PKC) isoforms contribute to the phosphorylating activity that modulates neurotransmitter release. In previous studies we showed that nPKCε is confined in the presynaptic site of the neuromuscular junction and its presynaptic function is activity-dependent. Furthermore, nPKCε regulates phorbol ester-induced acetylcholine release potentiation, which further indicates that nPKCε is involved in neurotransmission. The present study is designed to examine the nPKCε involvement in transmitter release at the neuromuscular junction. Results: We use the specific nPKCε translocation inhibitor peptide εV1-2 and electrophysiological experiments to investigate the involvement of this isoform in acetylcholine release. We observed that nPKCε membrane translocation is key to the synaptic potentiation of NMJ, being involved in several conditions that upregulate PKC isoforms coupling to acetylcholine (ACh) release (incubation with high Ca2+, stimulation with phorbol esters and protein kinase A, stimulation with adenosine 3′,5′-cyclic monophosphorothioate, 8-Bromo-, Rp-isomer, sodium salt -Sp-8-BrcAMP-). In all these conditions, preincubation with the nPKCε translocation inhibitor peptide (εV1-2) impairs PKC coupling to acetylcholine release potentiation. In addition, the inhibition of nPKCε translocation and therefore its activity impedes that presynaptic muscarinic autoreceptors and adenosine autoreceptors modulate transmitter secretion. Conclusions: Together, these results point to the importance of nPKCε isoform in the control of acetylcholine release in the neuromuscular junction.
    Áreas temáticas: Psicología Neurosciences Molecular biology Interdisciplinar Ciências biológicas ii Cellular and molecular neuroscience
    Acceso a la licencia de uso: https://creativecommons.org/licenses/by/3.0/es/
    Direcció de correo del autor: marta.tomas@urv.cat manuel.santafe@urv.cat mariaangel.lanuza@urv.cat josepmaria.tomas@urv.cat
    Identificador del autor: 0000-0002-4151-1697 0000-0002-5462-5108 0000-0003-4795-4103 0000-0002-0406-0006
    Fecha de alta del registro: 2023-03-11
    Versión del articulo depositado: info:eu-repo/semantics/publishedVersion
    Enlace a la fuente original: https://molecularbrain.biomedcentral.com/articles/10.1186/s13041-015-0171-5
    URL Documento de licencia: http://repositori.urv.cat/ca/proteccio-de-dades/
    Referencia al articulo segun fuente origial: Molecular Brain. 8 (1):
    Referencia de l'ítem segons les normes APA: Obis T; Hurtado E; Nadal L; Tomàs M; Priego M; Simon A; Garcia N; Santafe M; Lanuza M; Tomàs J (2015). The novel protein kinase C epsilon isoform modulates acetylcholine release in the rat neuromuscular junction. Molecular Brain, 8(1), -. DOI: 10.1186/s13041-015-0171-5
    DOI del artículo: 10.1186/s13041-015-0171-5
    Entidad: Universitat Rovira i Virgili
    Año de publicación de la revista: 2015
    Tipo de publicación: Journal Publications
  • Palabras clave:

    Cellular and Molecular Neuroscience,Molecular Biology,Neurosciences
    Transmitter release
    Skeletal-muscle
    Signal-transduction pathway
    Protein kinase c
    Protein kinase a
    Presynaptic muscarinic autoreceptors
    Pkc-epsilon
    Pkc epsilon
    Pkc
    Neurotransmitter release
    Neurotransmission
    Neuromuscular junction
    Muscarinic receptors
    Motor nerve terminal
    Induced cell-death
    Electrical stimulation
    Ca2+
    Ca 2+
    Anchoring proteins
    Adult-rat
    Adenosine receptors
    Ach release
    Acetylcholine release
    Psicología
    Neurosciences
    Molecular biology
    Interdisciplinar
    Ciências biológicas ii
    Cellular and molecular neuroscience
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