Autor según el artículo: Lozano-Bartolome, J; Llaurado, G; Rodriguez, M M; Fernandez-Real, J M; Garcia-Fontgivell, J F; Puig, J; Maymo-Masip, E; Vendrell, J; Chacon, M R
Departamento: Medicina i Cirurgia; Bioquímica i Biotecnologia
Autor/es de la URV: Maymo Masip, Elsa / Rodríguez Chacón, Matilde / Vendrell Ortega, Juan José
Palabras clave: Tweak; Triglycerides; Tnfsf12 protein, human; Tnf-alpha; Proinflammatory cytokine; Ppar-gamma; Obesity; Non-alcoholic fatty liver disease; Middle aged; Metabolic disease; Male; Liver; Insulin resistance; Humans; High cardiovascular risk; Hepatocytes; Hepatic insulin-resistance; Female; Fatty liver-disease; Cytokine tweak; Case-control studies; Body mass index; Bariatric surgery; Adult; Acid transporter cd36
Resumen: © 2016 Macmillan Publishers Limited. Context: Non-alcoholic fatty liver disease (NAFLD) is the hepatic manifestation of the metabolic syndrome and is strongly associated with obesity, dyslipidaemia and altered glucose regulation. Previous data demonstrated that low circulating levels of tumour necrosis factor weak inducer of apoptosis (sTWEAK) were associated with obesity, diabetes and insulin resistance, all traits associated with an increased risk of NALFD. Circulating sTWEAK levels are expected to be reduced in the presence of NAFLD. Objective: We aimed to explore the relationship between NAFLD and circulating sTWEAK levels in obese patients, and to evaluate the effect of sTWEAK on hepatocyte triglyceride accumulation. Design setting and patients: This is an observational case-control study performed in n=112 severely obese patients evaluated for NAFLD by abdominal ultrasound and n=32 non-obese patients without steatosis. Serum sTWEAK concentrations were measured by ELISA. Multivariable analyses were performed to determine the independent predictors of NAFLD. We analysed TWEAK and Fn14 protein expression in liver biopsies by western blotting and immunohistochemistry. An immortalized primary human hepatocyte cell line (HHL) was used to evaluate the effect of sTWEAK on triglyceride accumulation. Results: We observed a reduction in serum circulating sTWEAK concentrations with the presence of liver steatosis. On multivariable analysis, lower sTWEAK concentrations were independently associated with the presence of NAFLD (odds ratio (OR)=0.023; 95% confidence interval: 0.001-0.579; P<0.022). In human hepatocytes, sTWEAK administration reduced fat accumulation as demonstrated by the reduction in palmitic acid-induced accumulation of triglyceride and the decreased expression of cluster of differentiation 36 (CD36) and perilipin 1 and 2 (PLIN1 and PLIN2) genes. Conclusions: Decreased sTWEAK concentrations are independently associated with the presence of NAFLD. This is concordant with the observation that TWEAK reduces lipid accumulation in human liver cells.
Áreas temáticas: Serviço social; Saúde coletiva; Psicología; Nutrition and dietetics; Nutrition & dietetics; Nutrição; Medicine (miscellaneous); Medicina iii; Medicina ii; Medicina i; Interdisciplinar; General medicine; Farmacia; Enfermagem; Endocrinology, diabetes and metabolism; Endocrinology & metabolism; Educação física; Ciências biológicas ii; Ciências biológicas i; Ciência de alimentos; Biotecnología; Astronomia / física
Acceso a la licencia de uso: https://creativecommons.org/licenses/by/3.0/es/
ISSN: 03070565
Direcció de correo del autor: elsa.maymo@urv.cat; matilde.rodriguez@urv.cat; jvortega@iispv.cat
Fecha de alta del registro: 2025-02-01
Versión del articulo depositado: info:eu-repo/semantics/acceptedVersion
Enlace a la fuente original: https://www.nature.com/articles/ijo201673
URL Documento de licencia: https://repositori.urv.cat/ca/proteccio-de-dades/
Referencia al articulo segun fuente origial: International Journal Of Obesity. 40 (9): 1337-1345
Referencia de l'ítem segons les normes APA: Lozano-Bartolome, J; Llaurado, G; Rodriguez, M M; Fernandez-Real, J M; Garcia-Fontgivell, J F; Puig, J; Maymo-Masip, E; Vendrell, J; Chacon, M R (2016). Reduced circulating levels of sTWEAK are associated with NAFLD and may affect hepatocyte triglyceride accumulation. International Journal Of Obesity, 40(9), 1337-1345. DOI: 10.1038/ijo.2016.73
DOI del artículo: 10.1038/ijo.2016.73
Entidad: Universitat Rovira i Virgili
Año de publicación de la revista: 2016
Tipo de publicación: Journal Publications
Repositori URV