Articles producció científica> Ciències Mèdiques Bàsiques

Schizophyllum radiatum, an emerging fungus from human respiratory tract

  • Datos identificativos

    Identificador: imarina:6388875
    Autores:
    Siqueira JSutton DGené JGarcía DGuevara-Suarez MDecock CWiederhold NGuarro J
    Resumen:
    Copyright © 2016, American Society for Microbiology. All Rights Reserved. Schizophyllum is an important genus of basidiomycetes that, apart from being of genetic and biotechnological interest, is also reported to be a plant and animal pathogen. Schizophyllum commune is the best-known species and the only one reported from clinical specimens thus far, being recovered mainly from the respiratory tract. The aim of this study was to determine the species diversity of 23 clinical isolates of Schizophyllum from the United States using multilocus phylogenetic analysis and their in vitro susceptibilities to six drugs. The markers used for sequencing were the internal transcribed spacer (ITS), a portion of the nuclear large subunit (LSU) of ribosomal DNA, the RNA polymerase II second-largest subunit (RPB2), and the translation elongation factor 1α (EF-1α) gene. The analyses revealed that 22 of the clinical isolates were in the Schizophyllum radiatum clade with high support values and 1 isolate was in the S. commune clade. This is the first report of this species in clinical samples. The two species mentioned above showed very similar morphological features in culture (i.e., white, cottony, unsporulated colonies composed of hyphae with clamp connections), making morphological discrimination between the two impossible. An epitype is designed for S. radiatum, and its sequences have been deposited in GenBank. The antifungal that showed the greatest in vitro activity against the strains tested was shown to be amphotericin B. In general, the strains of S. radiatum showed higher MICs than S. commune.
  • Otros:

    Autor según el artículo: Siqueira J; Sutton D; Gené J; García D; Guevara-Suarez M; Decock C; Wiederhold N; Guarro J
    Departamento: Ciències Mèdiques Bàsiques
    Autor/es de la URV: García Sánchez, Dania / Gené Díaz, Josepa / Guarro Artigas, Josep / ZEN SIQUEIRA, JOAO PAULO
    Palabras clave: Rhinosinusitis Molecular characterization Infection Identification Epitypification Commune sinusitis Brain-abscess Basidiomycete Antifungal susceptibility Allergic bronchopulmonary mycosis
    Resumen: Copyright © 2016, American Society for Microbiology. All Rights Reserved. Schizophyllum is an important genus of basidiomycetes that, apart from being of genetic and biotechnological interest, is also reported to be a plant and animal pathogen. Schizophyllum commune is the best-known species and the only one reported from clinical specimens thus far, being recovered mainly from the respiratory tract. The aim of this study was to determine the species diversity of 23 clinical isolates of Schizophyllum from the United States using multilocus phylogenetic analysis and their in vitro susceptibilities to six drugs. The markers used for sequencing were the internal transcribed spacer (ITS), a portion of the nuclear large subunit (LSU) of ribosomal DNA, the RNA polymerase II second-largest subunit (RPB2), and the translation elongation factor 1α (EF-1α) gene. The analyses revealed that 22 of the clinical isolates were in the Schizophyllum radiatum clade with high support values and 1 isolate was in the S. commune clade. This is the first report of this species in clinical samples. The two species mentioned above showed very similar morphological features in culture (i.e., white, cottony, unsporulated colonies composed of hyphae with clamp connections), making morphological discrimination between the two impossible. An epitype is designed for S. radiatum, and its sequences have been deposited in GenBank. The antifungal that showed the greatest in vitro activity against the strains tested was shown to be amphotericin B. In general, the strains of S. radiatum showed higher MICs than S. commune.
    Grupo de investigación: Unitat de Micologia i Microbiologia Ambiental
    Áreas temáticas: Zootecnia / recursos pesqueiros Saúde coletiva Química Odontología Microbiology (medical) Microbiology Medicina veterinaria Medicina iii Medicina ii Medicina i Materiais Interdisciplinar Farmacia Ensino Engenharias ii Enfermagem Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Ciências ambientais Ciência de alimentos Ciência da computação Biotecnología Biodiversidade
    Acceso a la licencia de uso: https://creativecommons.org/licenses/by/3.0/es/
    ISSN: 00951137
    Direcció de correo del autor: dania.garcias@urv.cat josepa.gene@urv.cat
    Identificador del autor: 0000-0003-2397-1646 0000-0001-6195-9299
    Fecha de alta del registro: 2024-09-07
    Versión del articulo depositado: info:eu-repo/semantics/acceptedVersion
    Enlace a la fuente original: https://jcm.asm.org/content/54/10/2491.short
    URL Documento de licencia: https://repositori.urv.cat/ca/proteccio-de-dades/
    Referencia al articulo segun fuente origial: Journal Of Clinical Microbiology. 54 (10): 2491-2497
    Referencia de l'ítem segons les normes APA: Siqueira J; Sutton D; Gené J; García D; Guevara-Suarez M; Decock C; Wiederhold N; Guarro J (2016). Schizophyllum radiatum, an emerging fungus from human respiratory tract. Journal Of Clinical Microbiology, 54(10), 2491-2497. DOI: 10.1128/JCM.01170-16
    DOI del artículo: 10.1128/JCM.01170-16
    Entidad: Universitat Rovira i Virgili
    Año de publicación de la revista: 2016
    Tipo de publicación: Journal Publications
  • Palabras clave:

    Microbiology,Microbiology (Medical)
    Rhinosinusitis
    Molecular characterization
    Infection
    Identification
    Epitypification
    Commune sinusitis
    Brain-abscess
    Basidiomycete
    Antifungal susceptibility
    Allergic bronchopulmonary mycosis
    Zootecnia / recursos pesqueiros
    Saúde coletiva
    Química
    Odontología
    Microbiology (medical)
    Microbiology
    Medicina veterinaria
    Medicina iii
    Medicina ii
    Medicina i
    Materiais
    Interdisciplinar
    Farmacia
    Ensino
    Engenharias ii
    Enfermagem
    Ciências biológicas iii
    Ciências biológicas ii
    Ciências biológicas i
    Ciências ambientais
    Ciência de alimentos
    Ciência da computação
    Biotecnología
    Biodiversidade
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