Autor según el artículo: Siqueira J; Sutton D; Gené J; García D; Guevara-Suarez M; Decock C; Wiederhold N; Guarro J

Departamento: Ciències Mèdiques Bàsiques

Autor/es de la URV: García Sánchez, Dania / Gené Díaz, Josepa / Guarro Artigas, Josep / ZEN SIQUEIRA, JOAO PAULO

Palabras clave: Rhinosinusitis Molecular characterization Infection Identification Epitypification Commune sinusitis Brain-abscess Basidiomycete Antifungal susceptibility Allergic bronchopulmonary mycosis

Resumen: Copyright © 2016, American Society for Microbiology. All Rights Reserved. Schizophyllum is an important genus of basidiomycetes that, apart from being of genetic and biotechnological interest, is also reported to be a plant and animal pathogen. Schizophyllum commune is the best-known species and the only one reported from clinical specimens thus far, being recovered mainly from the respiratory tract. The aim of this study was to determine the species diversity of 23 clinical isolates of Schizophyllum from the United States using multilocus phylogenetic analysis and their in vitro susceptibilities to six drugs. The markers used for sequencing were the internal transcribed spacer (ITS), a portion of the nuclear large subunit (LSU) of ribosomal DNA, the RNA polymerase II second-largest subunit (RPB2), and the translation elongation factor 1α (EF-1α) gene. The analyses revealed that 22 of the clinical isolates were in the Schizophyllum radiatum clade with high support values and 1 isolate was in the S. commune clade. This is the first report of this species in clinical samples. The two species mentioned above showed very similar morphological features in culture (i.e., white, cottony, unsporulated colonies composed of hyphae with clamp connections), making morphological discrimination between the two impossible. An epitype is designed for S. radiatum, and its sequences have been deposited in GenBank. The antifungal that showed the greatest in vitro activity against the strains tested was shown to be amphotericin B. In general, the strains of S. radiatum showed higher MICs than S. commune.

Grupo de investigación: Unitat de Micologia i Microbiologia Ambiental

Áreas temáticas: Zootecnia / recursos pesqueiros Saúde coletiva Química Odontología Microbiology (medical) Microbiology Medicina veterinaria Medicina iii Medicina ii Medicina i Materiais Interdisciplinar Farmacia Ensino Engenharias ii Enfermagem Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Ciências ambientais Ciência de alimentos Ciência da computação Biotecnología Biodiversidade

Acceso a la licencia de uso: https://creativecommons.org/licenses/by/3.0/es/

ISSN: 00951137

Direcció de correo del autor: josep.guarro@urv.cat josepa.gene@urv.cat dania.garcias@urv.cat

Identificador del autor: 0000-0002-7839-7568 0000-0001-6195-9299 0000-0003-2397-1646

Fecha de alta del registro: 2023-05-21

Versión del articulo depositado: info:eu-repo/semantics/acceptedVersion

Enlace a la fuente original: https://jcm.asm.org/content/54/10/2491.short

URL Documento de licencia: http://repositori.urv.cat/ca/proteccio-de-dades/

Referencia al articulo segun fuente origial: Journal Of Clinical Microbiology. 54 (10): 2491-2497

Referencia de l'ítem segons les normes APA: Siqueira J; Sutton D; Gené J; García D; Guevara-Suarez M; Decock C; Wiederhold N; Guarro J (2016). Schizophyllum radiatum, an emerging fungus from human respiratory tract. Journal Of Clinical Microbiology, 54(10), 2491-2497. DOI: 10.1128/JCM.01170-16

DOI del artículo: 10.1128/JCM.01170-16

Entidad: Universitat Rovira i Virgili

Año de publicación de la revista: 2016

Tipo de publicación: Journal Publications