Autor según el artículo: Vegas C; Zavaleta AI; Canales PE; Esteve-Zarzoso B
Departamento: Bioquímica i Biotecnologia
e-ISSN: 2544-4646
Autor/es de la URV: Esteve Zarzoso, Braulio / VEGAS PÉREZ, CARLOS ALFREDO
Palabras clave: Yeast diversity Sp nov. Ribosomal-rna gene Rflp analysis Pcr-rflp L. Identification Fruit Fermentations Epiphytic yeasts Diversity Candida-pseudohaemulonii Bacteria Amazonia 5.8s-its
Resumen: Yeasts, commonly present on the surface of fruits, are of industrial interest for the production of enzymes, flavorings, and bioactive compounds, and have many other scientific uses. The Amazonian rainforest may be a good source of new species or strains of yeasts, but their presence on Amazonian fruits is unknown. The aim of this study was to identify and characterize yeasts isolated from Amazonian native fruits using molecular and phenotypic methods. In total, 81 yeast isolates were obtained from 10 fruits species. Rep-PCR showed 29 strain profiles. Using a combination of restriction-fragment length polymorphism (RFLP) of the 5.8S-ITS region and D1/D2 sequencing of the 26S rRNA gene, 16 species were identified belonging to genera Candida, Debaryomyces, Hanseniaspora, Kodamaea, Martiniozyma, and Meyerozyma. The most dominant species were Candida tropicalis, Debaryomyces hansenii, Hanseniaspora opuntiae, and Hanseniaspora thailandica. H. opuntiae and H. thailandica showed the highest number of the strain profiles. Phenotypic profiles were variable between species, and even among strains. Screening for hydrolases showed lipolytic activity in only one isolate, while proteolytic, cellulolytic and amylolytic capabilities were not detected. Yeast presence among fruits varied, with cidra (Citrus medica) and ungurahui (Oenocarpus bataua) having the highest number of species associated. This investigation broadens the understanding and possible biotechnological uses of yeast strains obtained from Amazonian native fruits. Yeasts, commonly present on the surface of fruits, are of industrial interest for the production of enzymes, flavorings, and bioactive compounds, and have many other scientific uses. The Amazonian rainforest may be a good source of new species or strains of yeasts, but their presence on Amazonian fruits is unknown. The aim of this study was to identify and characterize yeasts isolated from Amazonian native fruits using molecular and phenotypic methods. In total, 81 yeast isolates were obtained from 10 fruits species. Rep-PCR showed 29 strain profiles. Using a combination of restriction-fragment length polymorphism (RFLP) of the 5.8S-ITS region and D1/D2 sequencing of the 26S rRNA gene, 16 species were identified belonging to genera Candida, Debaryomyces, Hanseniaspora, Kodamaea, Martiniozyma, and Meyerozyma. The most dominant species were Candida tropicalis, Debaryomyces hansenii, Hanseniaspora opuntiae, and Hanseniaspora thailandica. H. opuntiae and H. thailandica showed the highest number of the strain profiles. Phenotypic profiles were variable between species, and even among strains. Screening for hydrolases showed lipolytic activity in only one isolate, while proteolytic, cellulolytic and amylolytic capabilities were not detected. Yeast presence among fruits varied, with cidra (Citrus medica) and ungurahui (Oenocarpus bataua) having the highest number of species associated. This investigation broadens the understanding and possible biotechnological uses of yeast strains obtained from Amazonian native fruits.
Áreas temáticas: Microbiology (medical) Microbiology Medicine (miscellaneous) General medicine Applied microbiology and biotechnology
Acceso a la licencia de uso: https://creativecommons.org/licenses/by/3.0/es/
ISSN: 1733-1331
Direcció de correo del autor: braulio.esteve@urv.cat braulio.esteve@urv.cat
Identificador del autor: 0000-0001-6467-7086 0000-0001-6467-7086
Fecha de alta del registro: 2024-07-27
Versión del articulo depositado: info:eu-repo/semantics/publishedVersion
Enlace a la fuente original: https://www.exeley.com/polish_journal_of_microbiology/doi/10.33073/pjm-2020-027
URL Documento de licencia: https://repositori.urv.cat/ca/proteccio-de-dades/
Referencia al articulo segun fuente origial: Polish Journal Of Microbiology. 69 (3): 1-11
Referencia de l'ítem segons les normes APA: Vegas C; Zavaleta AI; Canales PE; Esteve-Zarzoso B (2020). Yeasts Associated with Various Amazonian Native Fruits. Polish Journal Of Microbiology, 69(3), 1-11. DOI: 10.33073/pjm-2020-027
DOI del artículo: 10.33073/pjm-2020-027
Entidad: Universitat Rovira i Virgili
Año de publicación de la revista: 2020
Tipo de publicación: Journal Publications
Repositori URV