Autor según el artículo: Luciano-Mateo, Fedra; Cabre, Noemi; Fernandez-Arroyo, Salvador; Baiges-Gaya, Gerard; Hernandez-Aguilera, Anna; Rodriguez-Tomas, Elisabet; Munoz-Pinedo, Cristina; Menendez, Javier A.; Camps, Jordi; Joven, Jorge;
Departamento: Medicina i Cirurgia
Autor/es de la URV: Baiges Gaya, Gerard / Camps Andreu, Jorge / FERNANDEZ ARROYO, SALVADOR / Joven Maried, Jorge / Rodriguez Tomas, Elisabet
Palabras clave: Stress Monocyte chemoattractant protein-1 Metformin Mcp-1 Homeostasis Expression Ampk
Resumen: Chemokine (C-C motif) ligand 2 (CCL2) has been associated with chronic metabolic diseases. We aimed to investigate whether Ccl2 gene overexpression is involved in the regulation of signaling pathways in metabolic organs. Biochemical and histological analyses were used to explore tissue damage in cisgenic mice that overexpressed the Ccl2 gene. Metabolites from energy and one-carbon metabolism in liver and muscle extracts were measured by targeted metabolomics. Western blot analysis was used to explore the AMP-activated protein kinase (AMPK) and mammalian target of rapamycin pathways. Ccl2 overexpression resulted in steatosis, decreased AMPK activity and altered mitochondrial dynamics in the liver. These changes were associated with decreased oxidative phosphorylation and alterations in the citric acid cycle and transmethylation. In contrast, AMPK activity and its downstream mediators were increased in muscle, where we observed an increase in oxidative phosphorylation and increased concentrations of different metabolites associated with ATP synthesis. In conclusion, Ccl2 overexpression induces distinct metabolic alterations in the liver and muscle that affect mitochondrial dynamics and the regulation of energy sensors involved in cell homeostasis. These data suggest that CCL2 may be a therapeutic target in metabolic diseases.
Áreas temáticas: Zootecnia / recursos pesqueiros Saúde coletiva Química Psicología Odontología Nutrição Multidisciplinary sciences Multidisciplinary Medicina veterinaria Medicina iii Medicina ii Medicina i Materiais Matemática / probabilidade e estatística Letras / linguística Interdisciplinar Geografía Geociências Farmacia Engenharias iv Engenharias iii Engenharias ii Enfermagem Educação física Educação Economia Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Ciências ambientais Ciências agrárias i Ciência de alimentos Ciência da computação Biotecnología Biodiversidade Astronomia / física
Acceso a la licencia de uso: https://creativecommons.org/licenses/by/3.0/es/
Direcció de correo del autor: jorge.camps@urv.cat jorge.joven@urv.cat elisabet.rodriguezt@estudiants.urv.cat elisabet.rodriguezt@estudiants.urv.cat gerard.baiges@estudiants.urv.cat
Identificador del autor: 0000-0002-3165-3640 0000-0003-2749-4541
Fecha de alta del registro: 2023-08-05
Versión del articulo depositado: info:eu-repo/semantics/publishedVersion
Enlace a la fuente original: https://www.nature.com/articles/s41598-020-68769-7#article-info
Referencia al articulo segun fuente origial: Scientific Reports. 10 (1): 11954-
Referencia de l'ítem segons les normes APA: Luciano-Mateo, Fedra; Cabre, Noemi; Fernandez-Arroyo, Salvador; Baiges-Gaya, Gerard; Hernandez-Aguilera, Anna; Rodriguez-Tomas, Elisabet; Munoz-Pinedo (2020). Chemokine C-C motif ligand 2 overexpression drives tissue-specific metabolic responses in the liver and muscle of mice. Scientific Reports, 10(1), 11954-. DOI: 10.1038/s41598-020-68769-7
URL Documento de licencia: https://repositori.urv.cat/ca/proteccio-de-dades/
DOI del artículo: 10.1038/s41598-020-68769-7
Entidad: Universitat Rovira i Virgili
Año de publicación de la revista: 2020
Tipo de publicación: Journal Publications