Articles producció científica> Ciències Mèdiques Bàsiques

Microbial Signature in Adipose Tissue of Crohn's Disease Patients

  • Datos identificativos

    Identificador: imarina:8331758
    Autores:
    Serena, CarolinaQueipo-Ortuno, MaribelMillan, MonicaSanchez-Alcoholado, LidiaCaro, AleidisEspina, BeatrizMenacho, MargaritaBautista, MichelleMonfort-Ferre, DiandraTerron-Puig, MargaridaNunez-Roa, CatalinaMaymo-Masip, ElsaMar Rodriguez, M.Tinahones, Francisco J.Espin, EloyMarti, MarcFernandez-Veledo, SoniaVendrell, Joan
    Resumen:
    Crohn's disease (CD) is characterized by compromised immune tolerance to the intestinal commensal microbiota, intestinal barrier inflammation, and hyperplasia of creeping fat (CF) and mesenteric adipose tissue (AT), which seems to be directly related to disease activity. Gut microbiota dysbiosis might be a determining factor in CD etiology, manifesting as a low microbial diversity and a high abundance of potentially pathogenic bacteria. We tested the hypothesis that CF is a reservoir of bacteria through 16S-rRNA sequencing of several AT depots of patients with active and inactive disease and controls. We found a microbiome signature within CF and mesenteric AT from patients, but not in subcutaneous fat. We failed to detect bacterial DNA in any fat depot of controls. Proteobacteria was the most abundant phylum in both CF and mesenteric AT, and positively correlated with fecal calprotectin/C-reactive protein. Notably, the clinical status of patients seemed to be related to the microbiome signature, as those with the inactive disease showed a reduction in the abundance of pathogenic bacteria. Predictive functional profiling revealed many metabolic pathways including lipopolysaccharide biosynthesis and sulfur metabolism overrepresented in active CD relative to that in inactive CD. Our findings demonstrate that microbiota dysbiosis associated with CD pathophysiology is reflected in AT and might contribute to disease severity.
  • Otros:

    Autor según el artículo: Serena, Carolina; Queipo-Ortuno, Maribel; Millan, Monica; Sanchez-Alcoholado, Lidia; Caro, Aleidis; Espina, Beatriz; Menacho, Margarita; Bautista, Michelle; Monfort-Ferre, Diandra; Terron-Puig, Margarida; Nunez-Roa, Catalina; Maymo-Masip, Elsa; Mar Rodriguez, M.; Tinahones, Francisco J.; Espin, Eloy; Marti, Marc; Fernandez-Veledo, Sonia; Vendrell, Joan;
    Departamento: Ciències Mèdiques Bàsiques
    Autor/es de la URV: Fernandez Veledo, Sonia / Maymo Masip, Elsa / Menacho Viladot, Margarita / Monfort Ferre, Diandra / Serena Perelló, Carolina / Vendrell Ortega, Juan José
    Palabras clave: Ulcerative-colitis Tissue microbiota Stem-cells Picrust analysis Lipopolysaccharide biosynthesis Inflammatory bowel disease Inflammation Immune properties Ileostomy Gut microbiota Fusobacterium Fecal microbiota Escherichia coli Creeping fat Colorectal-cancer Colonic-mucosa 16s sequencing picrust analysis lipopolysaccharide biosynthesis inflammatory bowel disease fusobacterium escherichia coli creeping fat 16s sequencing
    Resumen: Crohn's disease (CD) is characterized by compromised immune tolerance to the intestinal commensal microbiota, intestinal barrier inflammation, and hyperplasia of creeping fat (CF) and mesenteric adipose tissue (AT), which seems to be directly related to disease activity. Gut microbiota dysbiosis might be a determining factor in CD etiology, manifesting as a low microbial diversity and a high abundance of potentially pathogenic bacteria. We tested the hypothesis that CF is a reservoir of bacteria through 16S-rRNA sequencing of several AT depots of patients with active and inactive disease and controls. We found a microbiome signature within CF and mesenteric AT from patients, but not in subcutaneous fat. We failed to detect bacterial DNA in any fat depot of controls. Proteobacteria was the most abundant phylum in both CF and mesenteric AT, and positively correlated with fecal calprotectin/C-reactive protein. Notably, the clinical status of patients seemed to be related to the microbiome signature, as those with the inactive disease showed a reduction in the abundance of pathogenic bacteria. Predictive functional profiling revealed many metabolic pathways including lipopolysaccharide biosynthesis and sulfur metabolism overrepresented in active CD relative to that in inactive CD. Our findings demonstrate that microbiota dysbiosis associated with CD pathophysiology is reflected in AT and might contribute to disease severity.
    Áreas temáticas: Medicine, general & internal Medicine (miscellaneous) Medicine (all)
    Acceso a la licencia de uso: https://creativecommons.org/licenses/by/3.0/es/
    ISSN: 20770383
    Direcció de correo del autor: margarita.menacho@urv.cat carolina.serena@urv.cat elsa.maymo@urv.cat diandra.monfort@estudiants.urv.cat sonia.fernandez@urv.cat juanjose.vendrell@urv.cat
    Identificador del autor: 0000-0002-9133-3120 0000-0003-3832-4249 0000-0003-2906-3788 0000-0002-6994-6115
    Fecha de alta del registro: 2024-09-07
    Versión del articulo depositado: info:eu-repo/semantics/publishedVersion
    Enlace a la fuente original: https://www.mdpi.com/2077-0383/9/8/2448
    URL Documento de licencia: https://repositori.urv.cat/ca/proteccio-de-dades/
    Referencia al articulo segun fuente origial: Journal Of Clinical Medicine. 9 (8): 1-16
    Referencia de l'ítem segons les normes APA: Serena, Carolina; Queipo-Ortuno, Maribel; Millan, Monica; Sanchez-Alcoholado, Lidia; Caro, Aleidis; Espina, Beatriz; Menacho, Margarita; Bautista, Mic (2020). Microbial Signature in Adipose Tissue of Crohn's Disease Patients. Journal Of Clinical Medicine, 9(8), 1-16. DOI: 10.3390/jcm9082448
    DOI del artículo: 10.3390/jcm9082448
    Entidad: Universitat Rovira i Virgili
    Año de publicación de la revista: 2020
    Tipo de publicación: Journal Publications
  • Palabras clave:

    Medicine (Miscellaneous),Medicine, General & Internal
    Ulcerative-colitis
    Tissue microbiota
    Stem-cells
    Picrust analysis
    Lipopolysaccharide biosynthesis
    Inflammatory bowel disease
    Inflammation
    Immune properties
    Ileostomy
    Gut microbiota
    Fusobacterium
    Fecal microbiota
    Escherichia coli
    Creeping fat
    Colorectal-cancer
    Colonic-mucosa
    16s sequencing
    picrust analysis
    lipopolysaccharide biosynthesis
    inflammatory bowel disease
    fusobacterium
    escherichia coli
    creeping fat
    16s sequencing
    Medicine, general & internal
    Medicine (miscellaneous)
    Medicine (all)
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