Articles producció científica> Enginyeria Electrònica, Elèctrica i Automàtica

Crosstalk between Drp1 phosphorylation sites during mitochondrial remodeling and their impact on metabolic adaptation

  • Datos identificativos

    Identificador: imarina:9226659
    Autores:
    Valera-Alberni, MiriamJoffraud, MagaliMiro-Blanch, JoanCapellades, JordiJunza, AlexandraDayon, LoicGalindo, Antonio NunezSanchez-Garcia, Jose LValsesia, ArmandCercillieux, AngeliqueSoellner, FlaviaLadurner, Andreas GYanes, OscarCanto, Carles
    Resumen:
    Mitochondria constantly undergo fusion and fission events, referred as mitochondrial dynamics, which determine mitochondrial architecture and bioenergetics. Cultured cell studies demonstrate that mitochondrial dynamics are acutely regulated by phosphorylation of the mitochondrial fission orchestrator dynamin-related protein 1 (Drp1) at S579 or S600. However, the physiological impact and crosstalk of these phosphorylation sites is poorly understood. Here, we describe the functional interrelation between S579 and S600 phosphorylation sites in vivo and their role on mitochondrial remodeling. Mice carrying a homozygous Drp1 S600A knockin (Drp1 KI) mutation display larger mitochondria and enhanced lipid oxidation and respiratory capacities, granting improved glucose tolerance and thermogenic response upon high-fat feeding. Housing mice at thermoneutrality blunts these differences, suggesting a role for the brown adipose tissue in the protection of Drp1 KI mice against metabolic damage. Overall, we demonstrate crosstalk between Drp1 phosphorylation sites and provide evidence that their modulation could be used in the treatment and prevention of metabolic diseases.
  • Otros:

    Autor según el artículo: Valera-Alberni, Miriam; Joffraud, Magali; Miro-Blanch, Joan; Capellades, Jordi; Junza, Alexandra; Dayon, Loic; Galindo, Antonio Nunez; Sanchez-Garcia, Jose L; Valsesia, Armand; Cercillieux, Angelique; Soellner, Flavia; Ladurner, Andreas G; Yanes, Oscar; Canto, Carles
    Departamento: Enginyeria Electrònica, Elèctrica i Automàtica
    Autor/es de la URV: Junza Martínez, Alexandra / Miró Blanch, Joan / Yanes Torrado, Óscar
    Palabras clave: Thermoneutrality Phosphorylation Pathway Morphology Mitochondrial respiration Mitochondrial dynamics Metabolic syndrome Liver protects Kinase Insulin-resistance Insulin resistance Fission factor Dynamin-related protein-1 Drp1 Degradation Deficiency Brown adipose tissue Adipocytes
    Resumen: Mitochondria constantly undergo fusion and fission events, referred as mitochondrial dynamics, which determine mitochondrial architecture and bioenergetics. Cultured cell studies demonstrate that mitochondrial dynamics are acutely regulated by phosphorylation of the mitochondrial fission orchestrator dynamin-related protein 1 (Drp1) at S579 or S600. However, the physiological impact and crosstalk of these phosphorylation sites is poorly understood. Here, we describe the functional interrelation between S579 and S600 phosphorylation sites in vivo and their role on mitochondrial remodeling. Mice carrying a homozygous Drp1 S600A knockin (Drp1 KI) mutation display larger mitochondria and enhanced lipid oxidation and respiratory capacities, granting improved glucose tolerance and thermogenic response upon high-fat feeding. Housing mice at thermoneutrality blunts these differences, suggesting a role for the brown adipose tissue in the protection of Drp1 KI mice against metabolic damage. Overall, we demonstrate crosstalk between Drp1 phosphorylation sites and provide evidence that their modulation could be used in the treatment and prevention of metabolic diseases.
    Áreas temáticas: Medicina ii Medicina i General medicine General biochemistry,genetics and molecular biology Engenharias iv Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Cell biology Biochemistry, genetics and molecular biology (miscellaneous) Biochemistry, genetics and molecular biology (all)
    Acceso a la licencia de uso: https://creativecommons.org/licenses/by/3.0/es/
    Direcció de correo del autor: oscar.yanes@urv.cat alexandra.junza@urv.cat joan.miro@estudiants.urv.cat
    Identificador del autor: 0000-0003-3695-7157 0000-0001-7205-0419 0000-0003-3179-0554
    Fecha de alta del registro: 2024-10-12
    Versión del articulo depositado: info:eu-repo/semantics/publishedVersion
    Enlace a la fuente original: https://www.cell.com/cell-reports/fulltext/S2211-1247(21)00999-2?_returnURL=https%3A%2F%2Flinkinghub.elsevier.com%2Fretrieve%2Fpii%2FS2211124721009992%3Fshowall%3Dtrue#
    URL Documento de licencia: https://repositori.urv.cat/ca/proteccio-de-dades/
    Referencia al articulo segun fuente origial: Cell Reports. 36 (8): 109565-
    Referencia de l'ítem segons les normes APA: Valera-Alberni, Miriam; Joffraud, Magali; Miro-Blanch, Joan; Capellades, Jordi; Junza, Alexandra; Dayon, Loic; Galindo, Antonio Nunez; Sanchez-Garcia, (2021). Crosstalk between Drp1 phosphorylation sites during mitochondrial remodeling and their impact on metabolic adaptation. Cell Reports, 36(8), 109565-. DOI: 10.1016/j.celrep.2021.109565
    DOI del artículo: 10.1016/j.celrep.2021.109565
    Entidad: Universitat Rovira i Virgili
    Año de publicación de la revista: 2021
    Tipo de publicación: Journal Publications
  • Palabras clave:

    Biochemistry, Genetics and Molecular Biology (Miscellaneous),Cell Biology
    Thermoneutrality
    Phosphorylation
    Pathway
    Morphology
    Mitochondrial respiration
    Mitochondrial dynamics
    Metabolic syndrome
    Liver protects
    Kinase
    Insulin-resistance
    Insulin resistance
    Fission factor
    Dynamin-related protein-1
    Drp1
    Degradation
    Deficiency
    Brown adipose tissue
    Adipocytes
    Medicina ii
    Medicina i
    General medicine
    General biochemistry,genetics and molecular biology
    Engenharias iv
    Ciências biológicas iii
    Ciências biológicas ii
    Ciências biológicas i
    Cell biology
    Biochemistry, genetics and molecular biology (miscellaneous)
    Biochemistry, genetics and molecular biology (all)
  • Documentos:

  • Cerca a google

    Search to google scholar