Autor según el artículo: Pehrsson M; Manon-Jensen T; Sun S; Villesen IF; Castañé H; Joven J; Patel K; Goodman Z; Nielsen MJ; Bay-Jensen AC; Leeming DJ; Mortensen JH; Karsdal MA
Departamento: Medicina i Cirurgia
Autor/es de la URV: Castañé Vilafranca, Helena / Joven Maried, Jorge
Palabras clave: Non-invasive biomarkers Hepatic fibrosis Fibrosis resolution Fatty liver-disease Collagen cross-linking sampling variability progression pro-c3 non-invasive biomarkers hepatic fibrosis fibrosis resolution
Resumen: Liver fibrosis results from a prolonged wound healing response to continued injury with excessive production of extracellular proteins. In patients with chronic liver disease, the monitoring of liver fibrosis dynamics is of high interest. While markers of fibrogenesis exist, markers of hepatic fibrosis resolution remain an unmet clinical need. Thus we sought to develop an assay quantifying a circulating proteolytic fragment of cross-linked type III collagen as a biomarker of fibrolysis, testing its utility in two clinical cohorts of liver fibrosis of distinct aetiology and regressing endotype.We used a monoclonal antibody targeting the C-telopeptide of type III collagen following C-proteinase cleavage to develop and validate a neo-epitope-specific enzyme-linked immunosorbent assay (CTX-III). A potential fibrosis resolution marker, CTX-III, was measured in two clinical cohorts of patients with obesity-associated non-alcoholic fatty liver disease undergoing bariatric surgery or hepatitis C virus infection from a clinical trial study evaluating the anti-fibrotic effect of farglitazar.CTX-III was robust and specific for the targeted neo-epitope with good reproducibility in EDTA plasma. We assessed type III collagen remodelling using a panel of biomarkers, including a type III collagen formation marker (PRO-C3), degradation (C3M), and CTX-III (fibrolysis). Net fibrolysis was increased in patients with non-alcoholic fatty liver disease following bariatric surgery (p<0.001). Moreover, net fibrolysis identified spontaneous fibrotic regressors from stable and progressors (p<0.05 and p<0.001) among hepatitis C virus infection patients.Circulating CTX-III as a marker of fibrolysis, indicating the biomarker's beneficial use in assessing hepatic fibrosis resolution.This article is protected by copyright. All rights reserved.
Áreas temáticas: Saúde coletiva Nutrição Medicina veterinaria Medicina iii Medicina ii Medicina i Interdisciplinar Hepatology General medicine Gastroenterology & hepatology Farmacia Engenharias iv Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Ciência de alimentos Biotecnología Astronomia / física
Acceso a la licencia de uso: https://creativecommons.org/licenses/by/3.0/es/
Direcció de correo del autor: helena.castane@estudiants.urv.cat jorge.joven@urv.cat
Identificador del autor: 0000-0003-2749-4541
Fecha de alta del registro: 2024-09-07
Versión del articulo depositado: info:eu-repo/semantics/publishedVersion
URL Documento de licencia: https://repositori.urv.cat/ca/proteccio-de-dades/
Referencia al articulo segun fuente origial: Liver International. 42 (7): 1605-1617
Referencia de l'ítem segons les normes APA: Pehrsson M; Manon-Jensen T; Sun S; Villesen IF; Castañé H; Joven J; Patel K; Goodman Z; Nielsen MJ; Bay-Jensen AC; Leeming DJ; Mortensen JH; Karsdal M (2022). An MMP-degraded and cross-linked fragment of type III collagen as a non-invasive biomarker of hepatic fibrosis resolution. Liver International, 42(7), 1605-1617. DOI: 10.1111/liv.15270
Entidad: Universitat Rovira i Virgili
Año de publicación de la revista: 2022
Tipo de publicación: Journal Publications
Repositori URV