Articles producció científica> Enginyeria Electrònica, Elèctrica i Automàtica

Electrochemical Biosensors Based on Convectively Assembled Colloidal Crystals

  • Datos identificativos

    Identificador: imarina:9271525
    Autores:
    Shiohara AEaston CDPrieto-Simon BVoelcker NH
    Resumen:
    Rapid, sensitive, selective and portable virus detection is in high demand globally. How-ever, differentiating non-infectious viral particles from intact/infectious viruses is still a rarely satisfied sensing requirement. Using the negative space within monolayers of polystyrene (PS) spheres deposited directly on gold electrodes, we fabricated tuneable nanochannels decorated with target-selective bioreceptors that facilitate the size-selective detection of intact viruses. Detection occurred through selective nanochannel blockage of diffusion of a redox probe, [Fe(CN)6]3/4−, allowing a quantifiable change in the oxidation current before and after analyte binding to the bioreceptor immobilised on the spheres. Our model system involved partial surface passivation of the mono-assembled PS spheres, by silica glancing angle deposition, to confine bioreceptor immobilisation specifically to the channels and improve particle detection sensitivity. Virus detection was first optimised and modelled with biotinylated gold nanoparticles, recognised by streptavidin immobilised on the PS layer, reaching a low limit of detection of 37 particles/mL. Intact, label-free virus detection was demonstrated using MS2 bacteriophage (~23–28 nm), a marker of microbiological contamination, showing an excellent limit of detection of ~1.0 pfu/mL. Tuneable nanochannel geometries constructed directly on sensing electrodes offer label-free, sensitive, and cost-efficient point-of-care biosensing platforms that could be applied for a wide range of viruses.
  • Otros:

    Autor según el artículo: Shiohara A; Easton CD; Prieto-Simon B; Voelcker NH
    Departamento: Enginyeria Electrònica, Elèctrica i Automàtica
    Autor/es de la URV: Prieto Simón, Beatriz
    Palabras clave: Virus detection Surface modification Electrochemical biosensors Convective assembly Colloidal crystal water virus detection pathogens nanoparticles convective assembly colloidal crystal beads bacteriophage-ms2
    Resumen: Rapid, sensitive, selective and portable virus detection is in high demand globally. How-ever, differentiating non-infectious viral particles from intact/infectious viruses is still a rarely satisfied sensing requirement. Using the negative space within monolayers of polystyrene (PS) spheres deposited directly on gold electrodes, we fabricated tuneable nanochannels decorated with target-selective bioreceptors that facilitate the size-selective detection of intact viruses. Detection occurred through selective nanochannel blockage of diffusion of a redox probe, [Fe(CN)6]3/4−, allowing a quantifiable change in the oxidation current before and after analyte binding to the bioreceptor immobilised on the spheres. Our model system involved partial surface passivation of the mono-assembled PS spheres, by silica glancing angle deposition, to confine bioreceptor immobilisation specifically to the channels and improve particle detection sensitivity. Virus detection was first optimised and modelled with biotinylated gold nanoparticles, recognised by streptavidin immobilised on the PS layer, reaching a low limit of detection of 37 particles/mL. Intact, label-free virus detection was demonstrated using MS2 bacteriophage (~23–28 nm), a marker of microbiological contamination, showing an excellent limit of detection of ~1.0 pfu/mL. Tuneable nanochannel geometries constructed directly on sensing electrodes offer label-free, sensitive, and cost-efficient point-of-care biosensing platforms that could be applied for a wide range of viruses.
    Áreas temáticas: Química Nanoscience & nanotechnology Medicine (miscellaneous) Materiais Instruments & instrumentation Instrumentation Engineering (miscellaneous) Clinical biochemistry Ciencias sociales Ciências biológicas iii Ciências biológicas i Chemistry, analytical Biotecnología Biotechnology Biomedical engineering Astronomia / física Analytical chemistry
    Acceso a la licencia de uso: https://creativecommons.org/licenses/by/3.0/es/
    Direcció de correo del autor: beatriz.prieto-simon@urv.cat
    Identificador del autor: 0000-0001-8016-1565
    Fecha de alta del registro: 2024-08-31
    Versión del articulo depositado: info:eu-repo/semantics/publishedVersion
    Enlace a la fuente original: https://www.mdpi.com/2079-6374/12/7/480
    URL Documento de licencia: https://repositori.urv.cat/ca/proteccio-de-dades/
    Referencia al articulo segun fuente origial: Biosensors. 12 (7):
    Referencia de l'ítem segons les normes APA: Shiohara A; Easton CD; Prieto-Simon B; Voelcker NH (2022). Electrochemical Biosensors Based on Convectively Assembled Colloidal Crystals. Biosensors, 12(7), -. DOI: 10.3390/bios12070480
    DOI del artículo: 10.3390/bios12070480
    Entidad: Universitat Rovira i Virgili
    Año de publicación de la revista: 2022
    Tipo de publicación: Journal Publications
  • Palabras clave:

    Analytical Chemistry,Biomedical Engineering,Biotechnology,Chemistry, Analytical,Clinical Biochemistry,Instrumentation,Instruments & Instrumentation,Medicine (Miscellaneous),Nanoscience & Nanotechnology
    Virus detection
    Surface modification
    Electrochemical biosensors
    Convective assembly
    Colloidal crystal
    water
    virus detection
    pathogens
    nanoparticles
    convective assembly
    colloidal crystal
    beads
    bacteriophage-ms2
    Química
    Nanoscience & nanotechnology
    Medicine (miscellaneous)
    Materiais
    Instruments & instrumentation
    Instrumentation
    Engineering (miscellaneous)
    Clinical biochemistry
    Ciencias sociales
    Ciências biológicas iii
    Ciências biológicas i
    Chemistry, analytical
    Biotecnología
    Biotechnology
    Biomedical engineering
    Astronomia / física
    Analytical chemistry
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