Autor según el artículo: Shiohara A; Easton CD; Prieto-Simon B; Voelcker NH
Departamento: Enginyeria Electrònica, Elèctrica i Automàtica
Autor/es de la URV: Prieto Simón, Beatriz
Palabras clave: Virus detection Surface modification Electrochemical biosensors Convective assembly Colloidal crystal water virus detection pathogens nanoparticles convective assembly colloidal crystal beads bacteriophage-ms2
Resumen: Rapid, sensitive, selective and portable virus detection is in high demand globally. How-ever, differentiating non-infectious viral particles from intact/infectious viruses is still a rarely satisfied sensing requirement. Using the negative space within monolayers of polystyrene (PS) spheres deposited directly on gold electrodes, we fabricated tuneable nanochannels decorated with target-selective bioreceptors that facilitate the size-selective detection of intact viruses. Detection occurred through selective nanochannel blockage of diffusion of a redox probe, [Fe(CN)6]3/4−, allowing a quantifiable change in the oxidation current before and after analyte binding to the bioreceptor immobilised on the spheres. Our model system involved partial surface passivation of the mono-assembled PS spheres, by silica glancing angle deposition, to confine bioreceptor immobilisation specifically to the channels and improve particle detection sensitivity. Virus detection was first optimised and modelled with biotinylated gold nanoparticles, recognised by streptavidin immobilised on the PS layer, reaching a low limit of detection of 37 particles/mL. Intact, label-free virus detection was demonstrated using MS2 bacteriophage (~23–28 nm), a marker of microbiological contamination, showing an excellent limit of detection of ~1.0 pfu/mL. Tuneable nanochannel geometries constructed directly on sensing electrodes offer label-free, sensitive, and cost-efficient point-of-care biosensing platforms that could be applied for a wide range of viruses.
Áreas temáticas: Química Nanoscience & nanotechnology Medicine (miscellaneous) Materiais Instruments & instrumentation Instrumentation Engineering (miscellaneous) Clinical biochemistry Ciencias sociales Ciências biológicas iii Ciências biológicas i Chemistry, analytical Biotecnología Biotechnology Biomedical engineering Astronomia / física Analytical chemistry
Acceso a la licencia de uso: https://creativecommons.org/licenses/by/3.0/es/
Direcció de correo del autor: beatriz.prieto-simon@urv.cat
Identificador del autor: 0000-0001-8016-1565
Fecha de alta del registro: 2024-08-31
Versión del articulo depositado: info:eu-repo/semantics/publishedVersion
Enlace a la fuente original: https://www.mdpi.com/2079-6374/12/7/480
URL Documento de licencia: https://repositori.urv.cat/ca/proteccio-de-dades/
Referencia al articulo segun fuente origial: Biosensors. 12 (7):
Referencia de l'ítem segons les normes APA: Shiohara A; Easton CD; Prieto-Simon B; Voelcker NH (2022). Electrochemical Biosensors Based on Convectively Assembled Colloidal Crystals. Biosensors, 12(7), -. DOI: 10.3390/bios12070480
DOI del artículo: 10.3390/bios12070480
Entidad: Universitat Rovira i Virgili
Año de publicación de la revista: 2022
Tipo de publicación: Journal Publications