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The Structure of Short and Genomic DNA at the Interparticle Junctions of Cationic Nanoparticles

  • Datos identificativos

    Identificador: imarina:9285544
    Autores:
    Gisbert-Quilis PMasetti MMorla-Folch JFitzgerald JMPazos-Perez NGarcia-Rico EGiannini VAlvarez-Puebla RAGuerrini L
    Resumen:
    Current understanding of the mechanisms underlying noncovalent interactions between native DNA and nanoparticles, as well as their impact on the double-helix structure, is far from providing a comprehensive view. It is known that these interactions are largely defined by the physicochemical properties of the metal/liquid interface, in particular by the nanoparticle surface charge. Remarkably, while DNA unzipping upon binding with cationic nanoparticles is reported, the exact determinants of this structural perturbation remain unclear. Herein, plasmon-based spectroscopies (surface-enhanced Raman scattering (SERS) and surface-plasmon resonance (SPR) and theoretical simulations are combined to directly investigate the role of the cooperative binding of cationic nanoparticles with different surface charges on the structural integrity of a large variety of DNAs. The intrinsic nature of the SERS effect unlocks the possibility of selectively examining the impact of nanoparticle clustering on the duplex structure over a wide degree of colloidal aggregation and without the need of external intercalating dyes or strand labeling. This extensive work provides new fundamental insights into the interaction between nucleic acids and nanoparticles, addressing key questions regarding the role played by multiple variables such as the nanoparticle surface charge, the DNA-mediated cluster size and geometry, and nucleic acids' length, composition, and conformational properties. © 2017 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
  • Otros:

    Autor según el artículo: Gisbert-Quilis P; Masetti M; Morla-Folch J; Fitzgerald JM; Pazos-Perez N; Garcia-Rico E; Giannini V; Alvarez-Puebla RA; Guerrini L
    Departamento: Química Física i Inorgànica
    Autor/es de la URV: Alvarez Puebla, Ramon Angel / Guerrini, Luca / Pazos Pérez, Nicolás Carlos
    Palabras clave: Tracer labels Surface-enhanced raman Surface functionality Submicroscopic particles Strand separation Spherical nucleic-acids Spectroscopy Nucleic acids Nanoparticles Highly fluorescent analogs Gold nanoparticles Enhanced raman-scattering Cytotoxicity Biological applications
    Resumen: Current understanding of the mechanisms underlying noncovalent interactions between native DNA and nanoparticles, as well as their impact on the double-helix structure, is far from providing a comprehensive view. It is known that these interactions are largely defined by the physicochemical properties of the metal/liquid interface, in particular by the nanoparticle surface charge. Remarkably, while DNA unzipping upon binding with cationic nanoparticles is reported, the exact determinants of this structural perturbation remain unclear. Herein, plasmon-based spectroscopies (surface-enhanced Raman scattering (SERS) and surface-plasmon resonance (SPR) and theoretical simulations are combined to directly investigate the role of the cooperative binding of cationic nanoparticles with different surface charges on the structural integrity of a large variety of DNAs. The intrinsic nature of the SERS effect unlocks the possibility of selectively examining the impact of nanoparticle clustering on the duplex structure over a wide degree of colloidal aggregation and without the need of external intercalating dyes or strand labeling. This extensive work provides new fundamental insights into the interaction between nucleic acids and nanoparticles, addressing key questions regarding the role played by multiple variables such as the nanoparticle surface charge, the DNA-mediated cluster size and geometry, and nucleic acids' length, composition, and conformational properties. © 2017 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
    Áreas temáticas: Química Mechanics of materials Mechanical engineering Materials science, multidisciplinary Materiais Interdisciplinar Chemistry, multidisciplinary Astronomia / física
    Acceso a la licencia de uso: https://creativecommons.org/licenses/by/3.0/es/
    Direcció de correo del autor: ramon.alvarez@urv.cat luca.guerrini@urv.cat nicolas.pazos@urv.cat
    Identificador del autor: 0000-0003-4770-5756 0000-0002-2925-1562 0000-0002-2326-4231
    Fecha de alta del registro: 2024-09-07
    Versión del articulo depositado: info:eu-repo/semantics/acceptedVersion
    Enlace a la fuente original: https://onlinelibrary.wiley.com/doi/abs/10.1002/admi.201700724
    URL Documento de licencia: https://repositori.urv.cat/ca/proteccio-de-dades/
    Referencia al articulo segun fuente origial: Advanced Materials Interfaces. 4 (17):
    Referencia de l'ítem segons les normes APA: Gisbert-Quilis P; Masetti M; Morla-Folch J; Fitzgerald JM; Pazos-Perez N; Garcia-Rico E; Giannini V; Alvarez-Puebla RA; Guerrini L (2017). The Structure of Short and Genomic DNA at the Interparticle Junctions of Cationic Nanoparticles. Advanced Materials Interfaces, 4(17), -. DOI: 10.1002/admi.201700724
    DOI del artículo: 10.1002/admi.201700724
    Entidad: Universitat Rovira i Virgili
    Año de publicación de la revista: 2017
    Tipo de publicación: Journal Publications
  • Palabras clave:

    Chemistry, Multidisciplinary,Materials Science, Multidisciplinary,Mechanical Engineering,Mechanics of Materials
    Tracer labels
    Surface-enhanced raman
    Surface functionality
    Submicroscopic particles
    Strand separation
    Spherical nucleic-acids
    Spectroscopy
    Nucleic acids
    Nanoparticles
    Highly fluorescent analogs
    Gold nanoparticles
    Enhanced raman-scattering
    Cytotoxicity
    Biological applications
    Química
    Mechanics of materials
    Mechanical engineering
    Materials science, multidisciplinary
    Materiais
    Interdisciplinar
    Chemistry, multidisciplinary
    Astronomia / física
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