Autor según el artículo: Gisbert-Quilis P; Masetti M; Morla-Folch J; Fitzgerald JM; Pazos-Perez N; Garcia-Rico E; Giannini V; Alvarez-Puebla RA; Guerrini L
Departamento: Química Física i Inorgànica
Autor/es de la URV: Alvarez Puebla, Ramon Angel / Guerrini, Luca / Pazos Pérez, Nicolás Carlos
Palabras clave: Tracer labels Surface-enhanced raman Surface functionality Submicroscopic particles Strand separation Spherical nucleic-acids Spectroscopy Nucleic acids Nanoparticles Highly fluorescent analogs Gold nanoparticles Enhanced raman-scattering Cytotoxicity Biological applications
Resumen: Current understanding of the mechanisms underlying noncovalent interactions between native DNA and nanoparticles, as well as their impact on the double-helix structure, is far from providing a comprehensive view. It is known that these interactions are largely defined by the physicochemical properties of the metal/liquid interface, in particular by the nanoparticle surface charge. Remarkably, while DNA unzipping upon binding with cationic nanoparticles is reported, the exact determinants of this structural perturbation remain unclear. Herein, plasmon-based spectroscopies (surface-enhanced Raman scattering (SERS) and surface-plasmon resonance (SPR) and theoretical simulations are combined to directly investigate the role of the cooperative binding of cationic nanoparticles with different surface charges on the structural integrity of a large variety of DNAs. The intrinsic nature of the SERS effect unlocks the possibility of selectively examining the impact of nanoparticle clustering on the duplex structure over a wide degree of colloidal aggregation and without the need of external intercalating dyes or strand labeling. This extensive work provides new fundamental insights into the interaction between nucleic acids and nanoparticles, addressing key questions regarding the role played by multiple variables such as the nanoparticle surface charge, the DNA-mediated cluster size and geometry, and nucleic acids' length, composition, and conformational properties. © 2017 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Áreas temáticas: Química Mechanics of materials Mechanical engineering Materials science, multidisciplinary Materiais Interdisciplinar Chemistry, multidisciplinary Astronomia / física
Acceso a la licencia de uso: https://creativecommons.org/licenses/by/3.0/es/
Direcció de correo del autor: ramon.alvarez@urv.cat nicolas.pazos@urv.cat luca.guerrini@urv.cat
Identificador del autor: 0000-0003-4770-5756 0000-0002-2326-4231 0000-0002-2925-1562
Fecha de alta del registro: 2023-02-23
Versión del articulo depositado: info:eu-repo/semantics/acceptedVersion
Enlace a la fuente original: https://onlinelibrary.wiley.com/doi/abs/10.1002/admi.201700724
Referencia al articulo segun fuente origial: Advanced Materials Interfaces. 4 (17):
Referencia de l'ítem segons les normes APA: Gisbert-Quilis P; Masetti M; Morla-Folch J; Fitzgerald JM; Pazos-Perez N; Garcia-Rico E; Giannini V; Alvarez-Puebla RA; Guerrini L (2017). The Structure of Short and Genomic DNA at the Interparticle Junctions of Cationic Nanoparticles. Advanced Materials Interfaces, 4(17), -. DOI: 10.1002/admi.201700724
URL Documento de licencia: http://repositori.urv.cat/ca/proteccio-de-dades/
DOI del artículo: 10.1002/admi.201700724
Entidad: Universitat Rovira i Virgili
Año de publicación de la revista: 2017
Tipo de publicación: Journal Publications
Repositori URV