Autor según el artículo: Chen S; Huang Z; Visalakshan RM; Liu H; Bachhuka A; Wu Y; Dabare PRL; Luo P; Liu R; Gong Z; Xiao Y; Vasilev K; Chen Z
Departamento: Enginyeria Electrònica, Elèctrica i Automàtica
Autor/es de la URV: Bachhuka, Akash
Palabras clave: Transepithelial medical devices Protein adsorption Plasma polymerization Epithelial barrier structure tantalum surfaces stability roughness protein adsorption plasma polymerization percutaneous devices merkel cells langerhans cells epithelial barrier structure cell-adhesion allylamine
Resumen: Transepithelial medical devices are increasing utilized in clinical practices. However, the damage of continuous natural epithelial barrier has become a major risk factor for the failure of epithelium-penetrating implants. How to increase the "epithelial barrier structures" (focal adhesions, hemidesmosomes, etc.) becomes one key research aim in overcoming this difficulty. Directly targeting the in situ "epithelial barrier structures" related proteins (such as fibronectin) absorption and functionalization can be a promising way to enhance interface-epithelial integration.Herein, we fabricated three plasma polymerized bio-interfaces possessing controllable surface chemistry. Their capacity to adsorb and functionalize fibronectin (FN) from serum protein was compared by Liquid Chromatography-Tandem Mass Spectrometry. The underlying mechanisms were revealed by molecular dynamics simulation. The response of gingival epithelial cells regarding the formation of epithelial barrier structures was tested.Plasma polymerized surfaces successfully directed distinguished protein adsorption profiles from serum protein pool, in which plasma polymerized allylamine (ppAA) surface favored adsorbing adhesion related proteins and could promote FN absorption and functionalization via electrostatic interactions and hydrogen bonds, thus subsequently activating the ITG β1-FAK-mTOR signaling and promoting gingival epithelial cells adhesion.This study offers an effective perspective to overcome the current dilemma of the inferior interface-epithelial integration by in situ protein absorption and functionalization, which may advance the development of functional transepithelial biointerfaces. Tuning the surface chemistry by plasma polymerization can control the adsorption of fibronectin and functionalize it by exposing functional protein domains. The functionalized fibronectin can bind to human gingival epithelial cell membrane integrins to activate epithelial barrier structure related signaling pathway, which eventually enhances the formation of epithelial barrier structure.© 2022. The Author(s).
Áreas temáticas: Medicine (miscellaneous) Materials science, biomaterials Engineering, biomedical Ceramics and composites Biomedical engineering Biomaterials
Acceso a la licencia de uso: https://creativecommons.org/licenses/by/3.0/es/
Direcció de correo del autor: akash.bachhuka@urv.cat
Identificador del autor: 0000-0003-1253-8126
Fecha de alta del registro: 2024-07-27
Versión del articulo depositado: info:eu-repo/semantics/publishedVersion
URL Documento de licencia: https://repositori.urv.cat/ca/proteccio-de-dades/
Referencia al articulo segun fuente origial: Biomater Res. 26 (1): 88-88
Referencia de l'ítem segons les normes APA: Chen S; Huang Z; Visalakshan RM; Liu H; Bachhuka A; Wu Y; Dabare PRL; Luo P; Liu R; Gong Z; Xiao Y; Vasilev K; Chen Z (2022). Plasma polymerized bio-interface directs fibronectin adsorption and functionalization to enhance "epithelial barrier structure" formation via FN-ITG β1-FAK-mTOR signaling cascade. Biomater Res, 26(1), 88-88. DOI: 10.1186/s40824-022-00323-0
Entidad: Universitat Rovira i Virgili
Año de publicación de la revista: 2022
Tipo de publicación: Journal Publications
Repositori URV