Autor según el artículo: Nieborak, Anna; Lukauskas, Saulius; Capellades, Jordi; Heyn, Patricia; Santos, Gabriela Silva; Motzler, Karsten; Zeigerer, Anja; Bester, Romina; Protzer, Ulrike; Schelter, Florian; Wagner, Mirko; Carell, Thomas; Hruscha, Alexander; Schmid, Bettina; Schneider, Robert
Departamento: Enginyeria Electrònica, Elèctrica i Automàtica
Autor/es de la URV: Yanes Torrado, Óscar
Palabras clave: Thioredoxin-interacting protein Ros Pyruvate kinase Metabolic flux Glycolysis Cancer Arrestins
Resumen: Cancer cells convert more glucose into lactate than healthy cells, what results in their growth advantage. Pyruvate kinase (PK) is a key rate limiting enzyme in this process, what makes it a promising potential therapeutic target. However, currently it is still unclear what consequences the inhibition of PK has on cellular processes. Here, we systematically investigate the consequences of PK depletion for gene expression, histone modifications and metabolism.Epigenetic, transcriptional and metabolic targets were analysed in different cellular and animal models with stable knockdown or knockout of PK.Depleting PK activity reduces the glycolytic flux and causes accumulation of glucose-6-phosphate (G6P). Such metabolic perturbation results in stimulation of the activity of a heterodimeric pair of transcription factors MondoA and MLX but not in a major reprogramming of the global H3K9ac and H3K4me3 histone modification landscape. The MondoA:MLX heterodimer upregulates expression of thioredoxin-interacting protein (TXNIP) - a tumour suppressor with multifaceted anticancer activity. This effect of TXNIP upregulation extends beyond immortalised cancer cell lines and is applicable to multiple cellular and animal models.Our work shows that actions of often pro-tumorigenic PK and anti-tumorigenic TXNIP are tightly linked via a glycolytic intermediate. We suggest that PK depletion stimulates the activity of MondoA:MLX transcription factor heterodimers and subsequently, increases cellular TXNIP levels. TXNIP-mediated inhibition of thioredoxin (TXN) can reduce the ability of cells to scavenge reactive oxygen species (ROS) leading to the oxidative damage of cellular structures including DNA. These findings highlight an important regulatory axis affecting tumour suppression mechanisms and provide an attractive opportunity for combination cancer therapies targeting glycolytic activity and ROS-generating pathways.Copyright © 2023 The Author(s). Published by Elsevier GmbH.. All rights reserved.
Áreas temáticas: Molecular biology Endocrinology & metabolism Ciências biológicas ii Cell biology
Acceso a la licencia de uso: https://creativecommons.org/licenses/by/3.0/es/
Direcció de correo del autor: oscar.yanes@urv.cat
Identificador del autor: 0000-0003-3695-7157
Fecha de alta del registro: 2024-10-12
Versión del articulo depositado: info:eu-repo/semantics/publishedVersion
URL Documento de licencia: https://repositori.urv.cat/ca/proteccio-de-dades/
Referencia al articulo segun fuente origial: Molecular Metabolism. 74 101748-101748
Referencia de l'ítem segons les normes APA: Nieborak, Anna; Lukauskas, Saulius; Capellades, Jordi; Heyn, Patricia; Santos, Gabriela Silva; Motzler, Karsten; Zeigerer, Anja; Bester, Romina; Protz (2023). Depletion of pyruvate kinase (PK) activity causes glycolytic intermediate imbalances and reveals a PK-TXNIP regulatory axis. Molecular Metabolism, 74(), 101748-101748. DOI: 10.1016/j.molmet.2023.101748
Entidad: Universitat Rovira i Virgili
Año de publicación de la revista: 2023
Tipo de publicación: Journal Publications
Repositori URV