Autor según el artículo: Paton, B; Herrero, P; Peraire, J; del Pino, A; Chafino, S; Martinez-Picado, J; Gómez-Bertomeu, F; Rull, A; Canela, N; Suárez, M
Departamento: Ciències Mèdiques Bàsiques Bioquímica i Biotecnologia
Autor/es de la URV: Gomez Bertomeu, Frederic-Francesc / HERRERO GIL, POL / Paton Jiménez, Beatrix / Peraire Forner, José Joaquin / RULL AIXA, ANNA / Suárez Recio, Manuel
Palabras clave: N-glycosylation Ms Lc-ms/ms Lc-ms Fucosylation Disease Covid-19 Biomarker
Resumen: BackgroundThe pathological mechanisms of SARS-CoV-2 in humans remain unclear and the unpredictability of COVID-19 progression may be attributed to the absence of biomarkers that contribute to the prognosis of this disease. Therefore, the discovery of biomarkers is needed for reliable risk stratification and to identify patients who are more likely to progress to a critical stage. MethodsAiming to identify new biomarkers we analysed N-glycan traits in plasma from 196 patients with COVID-19. Samples were classified into three groups according to their severity (mild, severe and critical) and obtained at diagnosis (baseline) and at 4 weeks of follow-up (postdiagnosis), to evaluate their behaviour through disease progression. N-glycans were released with PNGase F and labelled with Rapifluor-MS, followed by their analysis by LC-MS/MS. The Simglycan structural identification tool and Glycostore database were employed to predict the structure of glycans. ResultsWe determined that plasma from SARS-CoV-2-infected patients display different N-glycosylation profiles depending on the disease severity. Specifically, levels of fucosylation and galactosylation decreased with increasing severity and Fuc1Hex5HexNAc5 was identified as the most suitable biomarker to stratify patients at diagnosis and distinguish mild from critical outcomes. ConclusionIn this study we explored the global plasma glycosignature, reflecting the inflammatory state of the organs during the infectious disease. Our findings show the promising potential of glycans as biomarkers of COVID-19 severity.
Áreas temáticas: Zootecnia / recursos pesqueiros Saúde coletiva Química Odontología Nutrição Medicina veterinaria Medicina iii Medicina ii Medicina i Interdisciplinar Immunology and allergy Immunology Farmacia Engenharias iii Engenharias ii Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Ciência de alimentos Biotecnología Biodiversidade
Acceso a la licencia de uso: https://creativecommons.org/licenses/by/3.0/es/
Direcció de correo del autor: frederic-francesc.gomez@urv.cat joaquim.peraire@urv.cat beatrix.paton@estudiants.urv.cat manuel.suarez@urv.cat
Identificador del autor: 0000-0002-8039-2889 0000-0001-7808-5479 0000-0003-0122-8253
Fecha de alta del registro: 2024-08-03
Versión del articulo depositado: info:eu-repo/semantics/publishedVersion
Enlace a la fuente original: https://www.frontiersin.org/articles/10.3389/fimmu.2023.1204661/full
URL Documento de licencia: https://repositori.urv.cat/ca/proteccio-de-dades/
Referencia al articulo segun fuente origial: Frontiers In Immunology. 14
Referencia de l'ítem segons les normes APA: Paton, B; Herrero, P; Peraire, J; del Pino, A; Chafino, S; Martinez-Picado, J; Gómez-Bertomeu, F; Rull, A; Canela, N; Suárez, M (2023). Fucosylated N-glycans as early biomarkers of COVID-19 severity. Frontiers In Immunology, 14(), -. DOI: 10.3389/fimmu.2023.1204661
DOI del artículo: 10.3389/fimmu.2023.1204661
Entidad: Universitat Rovira i Virgili
Año de publicación de la revista: 2023
Tipo de publicación: Journal Publications