Autor según el artículo: Doerrier, C; Gnaiger, E; Gama-Perez, P; Garcia-Roves, PM; Pesta, D; Distefano, G; Goodpaster, BH; Coen, PM; Soendergaard, SD; Chroeis, KM; Larsen, S; Gonzalez-Franquesa, A; Prats, C; Sales-Pardo, M; Guimera, R
Departamento: Enginyeria Química
Autor/es de la URV: Guimera Manrique, Roger / Sales Pardo, Marta
Palabras clave: Small biopsies Responses Respiration medium z Respiration medium mir05 Phosphorylation Permeabilized skeletal muscle fibers Performance Oxphos protocols O2 regim O(2) regime O regime 2 Mitochondrial respiration Human-cells High-resolution respirometry H2o2 emission Exercise Capacit Blebbistatin Adaptations
Resumen: Aim: High-resolution respirometry in human permeabilized muscle fibers is extensively used for analysis of mitochondrial adaptions to nutrition and exercise interventions, and is linked to athletic performance. However, the lack of standardization of experimental conditions limits quantitative inter- and intra-laboratory comparisons. Methods: In our study, an international team of investigators measured mitochondrial respiration of permeabilized muscle fibers obtained from three biopsies ( vastus lateralis) from the same healthy volunteer to avoid inter-individual variability. High-resolution respirometry assays were performed together at the same laboratory to assess whether the heterogenity in published results are due to the effects of respiration media (MiR05 versus Z) with or without the myosin inhibitor blebbistatin at low- and high-oxygen regimes. Results: Our findings reveal significant differences between respiration media for OXPHOS and ETcapacities supported by NADH&succinate-linked substrates at different oxygen concentrations. Respiratory capacities were approximately 1.5-fold higher in MiR05 at high-oxygen regimes compared to medium Z near air saturation. The presence or absence of blebbistatin in human permeabilized muscle fiber preparations was without effect on oxygen flux. Conclusion: Our study constitutes a basis to harmonize and establish optimum experimental conditions for respirometric studies of permeabilized human skeletal muscle fibers to improve reproducibility.
Áreas temáticas: Zootecnia / recursos pesqueiros Química Psicología Physiology (medical) Odontología Nutrição Medicina veterinaria Medicina iii Medicina ii Medicina i Materiais Interdisciplinar General medicine Farmacia Engenharias iv Enfermagem Endocrinology & metabolism Educação física Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Ciências ambientais Ciência de alimentos Biotecnología Biodiversidade Biochemistry & molecular biology Biochemistry Astronomia / física
Acceso a la licencia de uso: https://creativecommons.org/licenses/by/3.0/es/
Direcció de correo del autor: roger.guimera@urv.cat marta.sales@urv.cat
Identificador del autor: 0000-0002-3597-4310 0000-0002-8140-6525
Fecha de alta del registro: 2024-09-14
Versión del articulo depositado: info:eu-repo/semantics/publishedVersion
Enlace a la fuente original: https://www.sciencedirect.com/science/article/pii/S0891584924005859?dgcid=rss_sd_all
Referencia al articulo segun fuente origial: Free Radical Biology And Medicine. 223 384-397
Referencia de l'ítem segons les normes APA: Doerrier, C; Gnaiger, E; Gama-Perez, P; Garcia-Roves, PM; Pesta, D; Distefano, G; Goodpaster, BH; Coen, PM; Soendergaard, SD; Chroeis, KM; Larsen, S; (2024). Harmonization of experimental procedures to assess mitochondrial respiration in human permeabilized skeletal muscle fibers. Free Radical Biology And Medicine, 223(), 384-397. DOI: 10.1016/j.freeradbiomed.2024.07.039
URL Documento de licencia: https://repositori.urv.cat/ca/proteccio-de-dades/
DOI del artículo: 10.1016/j.freeradbiomed.2024.07.039
Entidad: Universitat Rovira i Virgili
Año de publicación de la revista: 2024
Tipo de publicación: Journal Publications
Repositori URV