Autor según el artículo: Barranco-Altirriba, Maria; Alonso, Nuria; Weber, Ralf J M; Lloyd, Gavin R; Hernandez, Marta; Yanes, Oscar; Capellades, Jordi; Jankevics, Andris; Winder, Catherine; Falguera, Mireia; Franch-Nadal, Josep; Dunn, Warwick B; Perera-Lluna, Alexandre; Castelblanco, Esmeralda; Mauricio, Didac
Departamento: Enginyeria Electrònica, Elèctrica i Automàtica
Autor/es de la URV: Yanes Torrado, Óscar
Palabras clave: Mass-spectrometry Phospholipid Sex-specific difference Sex-specific differences Type 1 diabetes Type 2 diabetes Untargeted lipidomics
Resumen: Background In this study, we evaluated the lipidome alterations caused by type 1 diabetes (T1D) and type 2 diabetes (T2D), by determining lipids significantly associated with diabetes overall and in both sexes, and lipids associated with the glycaemic state.Methods An untargeted lipidomic analysis was performed to measure the lipid profiles of 360 subjects (91 T1D, 91 T2D, 74 with prediabetes and 104 controls (CT)) without cardiovascular and/or chronic kidney disease. Ultra-high performance liquid chromatography-electrospray ionization mass spectrometry (UHPLC-ESI-MS) was conducted in two ion modes (positive and negative). We used multiple linear regression models to (1) assess the association between each lipid feature and each condition, (2) determine sex-specific differences related to diabetes, and (3) identify lipids associated with the glycaemic state by considering the prediabetes stage. The models were adjusted by sex, age, hypertension, dyslipidaemia, body mass index, glucose, smoking, systolic blood pressure, triglycerides, HDL cholesterol, LDL cholesterol, alternate Mediterranean diet score (aMED) and estimated glomerular filtration rate (eGFR); diabetes duration and glycated haemoglobin (HbA1c) were also included in the comparison between T1D and T2D.Results A total of 54 unique lipid subspecies from 15 unique lipid classes were annotated. Lysophosphatidylcholines (LPC) and ceramides (Cer) showed opposite effects in subjects with T1D and subjects with T2D, LPCs being mainly up-regulated in T1D and down-regulated in T2D, and Cer being up-regulated in T2D and down-regulated in T1D. Also, Phosphatidylcholines were clearly down-regulated in subjects with T1D. Regarding sex-specific differences, ceramides and phosphatidylcholines exhibited important diabetes-associated differences due to sex. Concerning the glycaemic state, we found a gradual increase of a panel of 1-deoxyceramides from normoglycemia to prediabetes to T2D.Conclusions Our findings revealed an extensive disruption of lipid metabolism in both T1D and T2D. Additionally, we found sex-specific lipidome changes associated with diabetes, and lipids associated with the glycaemic state that can be linked to previously described molecular mechanisms in diabetes.
Áreas temáticas: Biotecnología Cardiac & cardiovascular systems Cardiology and cardiovascular medicine Ciências biológicas i Ciências biológicas ii Educação física Endocrinology & metabolism Endocrinology, diabetes and metabolism Farmacia Interdisciplinar Internal medicine Medicina i Medicina ii Saúde coletiva
Acceso a la licencia de uso: https://creativecommons.org/licenses/by/3.0/es/
Direcció de correo del autor: oscar.yanes@urv.cat
Identificador del autor: 0000-0003-3695-7157
Fecha de alta del registro: 2024-10-12
Versión del articulo depositado: info:eu-repo/semantics/publishedVersion
Referencia al articulo segun fuente origial: Cardiovascular Diabetology. 23 (1): 109-
Referencia de l'ítem segons les normes APA: Barranco-Altirriba, Maria; Alonso, Nuria; Weber, Ralf J M; Lloyd, Gavin R; Hernandez, Marta; Yanes, Oscar; Capellades, Jordi; Jankevics, Andris; Winde (2024). Lipidome characterisation and sex-specific differences in type 1 and type 2 diabetes mellitus. Cardiovascular Diabetology, 23(1), 109-. DOI: 10.1186/s12933-024-02202-5
URL Documento de licencia: https://repositori.urv.cat/ca/proteccio-de-dades/
Entidad: Universitat Rovira i Virgili
Año de publicación de la revista: 2024
Tipo de publicación: Journal Publications
Repositori URV