Repositori URV

Identificador: TDX:770

Handle: https://hdl.handle.net/20.500.11797/TDX770

Autors:
Brossa Piñero, Lidia

Resum:
The scope of this thesis is to develop analytical methods to determine endocrine disruptors and to control their presence in environmental water samples. Some methods are based on gas chromatography-mass spectrometry (GC-MS) using several extraction techniques such as solid-phase extraction (SPE) and the novel technique, stir-bar sorptive extraction (SBSE). These methods have been developed by using large volume injection (LVI) techniques to introduce the extract into the GC system. The SPE has been coupled to GC using the on-column interface and a programmable temperature vaporizer (PTV), to increase both automation and sensibility. SBSE with liquid desorption has been used, instead of thermal desorption (the most used in combination with GC) to study its applicability with LVI.Methods based on high-performance liquid chromatography (HPLC) combined with SPE as a preconcentration technique, have been developed to increase the number of analytes studied. In these methods UV detector has been used to obtain a rapid method that can be easily applied as a control method in the routine laboratories. MS with electrospray interface has also been used. Some of the developed methods have been used to monitor several groups of endocrine disruptors in environmental water samples from river, sea and influent and effluent sewage treatment plants. These samples have been collected in Tarragona, where no studies related to the control of these compounds have been done.