DNA biosensors based on integrated isothermal amplification-detection strategies

Identifier:  TDX:2470

Handle: https://hdl.handle.net/20.500.11797/TDX2470

Authors:  Sabaté del Río, Jonathan

Abstract:
The main goal of this Doctoral Thesis is to present alternative approaches in the field of DNA biosensors, designing and building new detection platforms that combine the amplification and quantification of targeted DNA while overcoming some of the current limitations. In order to achieve this objective, a variety of different strategies of effective solid-phase immobilisation strategies and isothermal enzymatic amplification have been explored to achieve lower detection limits with rapid and easy to execute assays. This work presents a convenient, rapid, simple, easy to integrate yet robust biosensing detection platform that can bring new ideas for the integration of nucleic acid tests in point of care devices through the use of lab on a chip solutions. Ultimately, the vision underlining this work is to humbly contribute to the concept of decentralisation, allowing molecular diagnostics to move away from laboratories, providing bioanalytical information in situ. The Thesis report the work performed to achieve the specific objectives of this Doctoral Thesis: the use of solid-phase recombinase polymerase amplification strategy, as a hybrid concept that combines DNA amplification and detection, the detection of real samples, the exploiting of the surface chemistry and DNA to overcome the limitations found, and the use of ring resonators for the label-free and real-time monitoring of the RPA mechanism.