Protheomic and adipo/cytokine biomarker analysis of unstable carotid atheroma plaque

Identifier:  TDX:3968

Handle: https://hdl.handle.net/20.500.11797/TDX3968

Authors:  Alibalic, Ajla

Abstract:
Arteriosclerosis is prevalent pathological process that causes clinical manifestations of vascular diseases such as myocardial infarction, stroke or peripheral arterial occlusive disease. Objectives of this thesis are to identify potential candidate biomarkers for carotid atherosclerosis (CA) in secretome samples of patients with unstable carotid plaque and to study the possible role of some adipo/cytokines in the stability of atherosclerotic plaques. We also analysed the levels of several pro- and anti-inflammatory adipo/cytokines in serum and unstable plaque secretome according to the presence of cardiovascular risk factors. In a first study, we performed proteomic quantitative analysis in secretome samples obtained from human carotid atherosclerotic plaques by carotid endarterectomy. In a second, we analysed the levels of several pro- and anti-inflammatory adipo/cytokines in serum and unstable plaque secretome by enzyme-linked immunosorbent assays (ELISA). Some proteomic changes were detected in the secretomes of carotid atherosclerotic plaques (CAP) compared with non atherosclerotic mammary arteries. The increased proteins in CAP secretomes were neutrophil defensin 1, apolipoprotein E, clusterin and zinc-alpha-2-glycoprotein.