A multi-omics approach to describe crosstalk between gut microbiota and host: epigenetic and virome role

Identifier:  TDX:4162

Handle: https://hdl.handle.net/20.500.11797/TDX4162

Authors:  Kazakova Borodina, Polina

Abstract:
The gut microbiota plays a crucial role in maintaining human health and homeostasis. Therefore, the development of gut microbiota during early life significantly impacts later health. Dysbiosis, or changes in the gut microbiota composition, has been associated with long-term health disorders such as celiac disease. However, while the bacterial microbiota has been extensively studied, the virome, which is much more diverse than all other kingdoms, is relatively less understood. Transkingdom interplay between bacteria and viruses impacts host health beyond their individual effects, emphasizing the importance of exploring this relationship. To gain further insight into the relationship between the microbiota and its host in early life stages and in the context of childhood celiac disease, this doctoral thesis aims to describe the microbiota of meconium and stool samples from milk-fed infants and to compare the possible alterations of healthy children with that of children with celiac disease adhering to a gluten-free diet. Additionally, it assesses the effect of probiotic administration in an animal model of celiac disease. By utilizing a combination of different omics sciences, this thesis has the potential to uncover valuable insights into the functionality and activity of the intestinal microbiota and its relationship with diseases. The research in this thesis will contribute to a better understanding of the significance of transkingdom interplay and its roles in host homeostasis and health conditions.