Next generation molecular diagnostic electrochemical platforms

Identifier:  TDX:4530

Handle: https://hdl.handle.net/20.500.11797/TDX4530

Authors:  Yenice, Cansu Pinar

Abstract:
Electrochemical detection is particularly attractive for biosensors due to the high sensitivity, simplicity, rapid response, cost-effectiveness and simplified instrumentation as compared to that required for optical transduction, as well as its compatibility with miniaturization and low sample volumes. Although the intrinsic redox properties of all nucleobases of DNA and RNA enable the development of label-free electrochemical biosensors to provide structure-sensitive DNA analysis and/or detection analysis with the advantage of direct DNA measurement, they face limitations with regards to the choice of electrode material, lack of sensitivity or the ability to achieve regeneratable sensors. External electroactive labels have been introduced not only to overcome these limitations but also to improve the sensitivity of sensors and facilitate the use of a greater range of electrode materials. The first part of this thesis explores the use of redox labels, moving towards a final goal of achieving an approach for electrochemical DNA sequencing. Successful incorporation of five oxidizable redox labels achieved simultaneous and ratiometric electrochemical detection and the quantification of the total number of each nucleobases was achieved.