Susceptibilidad antifúngica y filogenia molecular de especies del género fusarium de interés clínico

Identifier:  TDX:968

Handle: https://hdl.handle.net/20.500.11797/TDX968

Authors:  Azor Heras, Mónica

Abstract:
Summary: “Antifungal susceptibility and molecular phylogeny of the genus fusarium of clinical interest” Introduction. Fusarium solani, Fusarium oxysporum and Fusarium verticillioides, as well as other less frequent species of the genus, are important opportunists pathogenic to Man for which there is still no effective treatment. In addition, sensitivity to effective antifungal treatments varies depending on species, therefore identification of samples to species level is of primary interest. However, identification on the basis of morphological characteristics can be difficult. New techniques in molecular biology have made identification easier and allowed more adequate criteria to be established in order to delimit the species of the genus. Aims. To select better genetic markers for the molecular identification of the species of the genus, to determine the existence of phylogenetic groups that can delimit samples of each of the species identified, to evaluate the in vitro activity of different antifungals against species of Fusarium of clinical interest, and to find out if there are any differences with respect to the antifungal sensitivity of the phylogenetic groups obtained. Findings. Multigenetic analysis carried out with samples of F.solani have confirmed that it is a species complex that presents a high resistance against all the antifungals tested. The analysis of gene EF-1α has confirmed the identification of four phylogenetic groups within the F.oxysporum complex that have shown different antifungal sensitivity in vitro against terbinafine. The TUB region of the gene of β-tubulina has proven to be an excellent molecular marker for identifying the other species of clinical interest that have shown sensitivity primarily against terbinafine.