Evidències en teixit adipós d'alteracions gèniques dels vasos limfàtics en l'obesitat

Identificador:  TDX:2237

Handle: https://hdl.handle.net/20.500.11797/TDX2237

Autores:  Horra Pueyo, Alfons

Resumen:
Obesity is characterized by an excessive adipose tissue accumulation both in subcutaneous and visceral depots. Systemic and local events, take part in this morbidity. Interstitial drainage by lymphatic vessel in adipose tissue is one of the structural modifications, that has been scarcely considered up to now. In our study we have screened adipose tissue expression of several genes linked with lymphatic vessels in different ways, by means of microarray analyses and RT PCR gene expression studies in several cohorts, including lean, obese and patients with insulin resistance and type 2 diabetes mellitus. We have hypothesized that differences could be found considering the diverse metabolic dysfunctions mentioned above. Joined analysis of the two main genes involved in the lymphatic context; prox1 and pdpn, allows us to establish an indirect link between obesity and modifications of the adipose tissue gene expression, both in subcutaneous and visceral fat. A higher mRNA expression of both genes, is observed in visceral fat in all of the studied metabolic conditions. Likewise, we describe a number of associations with clinical, analytical and gene expression variables regarding these two selected genes, being the autophagy related 7 (atg7) and autophagy related 3 (atg3) mRNA gene expression the main determinants of prox1 and pdn expression in adipose tissue, respectively. We conclude that hydrodynamic dysfunction could be considered as a new potential player in adipose tissue mainly in obese people. New mechanistic studies are guaranteed.