Aplicación de corrientes galvánicas intramusculares en ratones con puntos gatillo miofasciales

Identificador:  TDX:3257

Handle: https://hdl.handle.net/20.500.11797/TDX3257

Autores:  Margalef Perez, Joan Ramon

Resumen:
The myofascial trigger point (MTrPs) is one of those responsible for generating pain in patients. According to the integrated hypothesis, an elevated spontaneous neurotransmission in one or more neuromuscular junctions (NMJ) may be involved. In this thesis, acetylcholinesterase inhibitor drugs have generated an increase in acetylcholine (ACh) in the synaptic cleft of the NMJin muscles of Swiss mice and Sprague Dawley rats. Through intracellular recordings and electromyographic recordings, a large increase in spontaneous neurotransmission was observed. These changes are associated with a decrease in the area of ACh receptors, the presence of contracted nodes near the NMJ and an increase in glycosaminoglycans. In addition, local twitch responses (LTR) were identified by ultrasound after stimulating the MTrPs with a needle. With this animal model of MTrPs, galvanic currents (GC) were applied intramuscularly through needles. GC with the protocols 1.5m for 5 seconds and 3 applications, and 3 mA for 3 seconds and 3 applications were the most effective in reversing the electrophysiological recordings. Finally, the CG generated a greater number of LTR and each of greater speed. It has been described that the administration of GC through solid needles in biological tissues can generate an increase in temperature, a deterioration of the morphology and compounds of the needles. Therefore, 3 mA for 3 seconds and 3 applications of Physio Invasiva® and AguPunt® needles were evaluated in tubes filled with Ringer's solution and others inserted into muscles. The results did not show changes in temperature or pH, my resistance, nor in composition and morphology. However, if they can cause deterioration of the morphology and composition of the materials, if the number of applications is increased.