Multivariate curve resolution applied to sequential injection data. Analysis of amoxicillin anda clavulanic acid

Identificador:  TDX:783

Handle: https://hdl.handle.net/20.500.11797/TDX783

Autores:  Pasamontes Fúnez, Alberto

Resumen:
The objective of this thesis is to study and develop analytical methods to determineamoxicillin and clavulanic acid in pharmaceuticals using sequential injection analysis (SIA)with a diode-array spectrophotometric detector to obtain second-order data. To treat thesedata, the chemometric tool used was; multivariate curve resolution with alternating leastsquares (MCR-ALS) and the techniques involved in the resolution process are: principalanalysis components (PCA) and simple-to-use interactive self-modelling mixture analysis(SIMPLISMA).The first chapter contains a brief description of the theoretical backgrounds thathave been used during this thesis. We explain the characteristics and properties ofamoxicillin and clavulanic acid, we describes the instrumental and the chemometric toolsused and at the end, we introduce the experimental designs used and the desirabilityfunction.In the next two chapters contain the bulk of the work carried out for this thesis andincorporate papers published in journals. In the first paper, the pharmaceuticals wereclassified according to their selective zones in order to propose the type of calibration. In aprevious step, the experimental work was conducted to find an analytical sequence thatallows us to obtain an evolving system. This step was carried out using experimentaldesign. In the second paper, the quantity of amoxicillin in the pharmaceuticals withinterferents or without selective zones was determined. To carry out correctly thecalibration step, we studied different conditions related to the MCR-ALS process.In the third paper, we propose the simultaneous determination of amoxicillin andclavulanic acid which they have the acid-base characteristics and spectral profile similar.To determine both analytes, a new analytical sequence was re