Comparison of chromatographic separation methods in lipidomics: with or without HPTLC

Identifier:  TFG:115

Handle: https://hdl.handle.net/20.500.11797/TFG115

Authors:  Fumadó Roig, Sonia

Abstract:
Lipodomics is a booming science. It’s a part of metabolomics and it consists in lipid analysis. It develops in three steps: extraction, separation and analysis of lipids. For chromatographic separation we used HPTLC and HPLC and for analysis we used Q-TOF and QqQ. Phospholipids are a type of lipid that have important functions such as the formation of cell membranes, signal transmission or the formation of lipoproteins. Our objective was to determine if the application of HPTLC before HPLC separation implies an improvement in the detection of phospholipids. We used various working conditions: five different extractions, eight mobile phase for HPTLC, with and without derivatization process and using a volume of 5μl and 50μl in a HPTLC plate. We have concluded that it is not necessary because the results for analysis are the same.