Development of electrochemical immunosensors based on the use of magnetic beads for in situ detection of IL-6 and TNFa

Identifier:  TFG:1642

Handle: https://hdl.handle.net/20.500.11797/TFG1642

Authors:  Raigón López, Sofía

Abstract:
In recent years, experiments developed using biosensors have increased exponentially. A Scopus study in the publication period between 1972-2017 using the word biosensor reveals an exponential trend in the number of publications in this field. This is due to the multiple applications offered by biosensors, which are used in biology, analytical chemistry, physics and bioelectronics. In this project we bet on the development of electrochemical transduction immunosensors using magnetic beads, using screen-printed carbon electrodes, to detect IL-6 and TNFα. The integration of magnetic beads in bioanalytical assays allows efficient capture and separation of the analyte. In addition, a large amplification of the signal can be achieved with the use of enzymes. The main objective of this work focuses on the development of an in situ capture system of the cytokines IL6 and TNFα, using magnetic beads functionalized with specific antibodies, in order to study the different effects on the tissue, both of physical and chemical stimuli. To achieve this goal, we worked on the optimization of the concentration of each immunoreagent and the different test conditions, for the construction of the calibration curves for each of the biomarkers.