Changes in NMR lipoprotein subclass profile after manual lymphatic drainage

Identifier:  TFG:1780

Handle: https://hdl.handle.net/20.500.11797/TFG1780

Authors:  Balsells Llauradó, Clara

Abstract:
During the last few years, many studies have described a close connection between blood and lymph with an exchange of fluids between both systems. For that reason, our study is set to determine whether there is a variation on plasma lipoprotein concentration before and after performing a lymphatic drainage massage (LDM). The study included 25 apparently healthy young men older than 18 years old. The volunteers were submitted to a blood extraction, the LDM procedure followed by a second blood extraction. The LDM started in the lower extremities, followed by the anterior part of the trunk and ended in the upper extremities. In total, the massage lasted for 35 minutes. Fasting venous blood samples were collected in EDTA tubes and centrifuged to obtain the plasma. To measure lipoprotein particle size, diffusion-ordered NMR spectroscopy (DOSY) was used. Results show significant changes on triglycerides but not in cholesterol concentrations. Individuals consistently display a significantly decrease in VLDL-related parameters (cholesterol, triglycerides and number of particles). The effect on VLDL is diameter-dependent as we have observed that the smaller the VLDL particle the larger the decrease associated with LDM. In summary, we have confirmed the hypothesis that LDM modulates circulating lipoprotein concentration, being this effect associated with TG-rich lipoproteins.