Identifier: TFG:2136
Authors: Vera Gómez, Iván
Abstract:
Karlodinium armiger is a toxic microalgae which affects coastal ecosystems by releasing a toxin. For this reason, a Karlodinium detection system is needed. Although there have already been developed some detection methods, those are expensive, and they need specialized workers. In order to avoid these problems, it’s necessary to develop an ASSURED biosensor (Affordable, Sensitive, Specific, User-friendly, Rapid and Robust, Equipment free, Deliverable to all end-users). The first step in the biosensor development is to set up a basic standard detection method for basing the following studies. One of the detection methods is RPA-ELONA. However, actually the ELONA assays are done in maleimide plates, which make this method expensive and complex. Due to this, this project will work in the development of a new RPA-ELONA using plates with poly-l-lysine (PLL). This new system would be cheaper, because of the price of the plates and the reagents needed. The results obtained here demonstrate this system can detect K. armiger DNA nearly like maleimide plates did. Therefore, this detection system it’s very promising. Nevertheless, more studies should optimize this system making it more sensitive. Key words: ASSURED, RPA, PLL , ELONA, Maleimide, Karlodinium armiger
Repositori URV