Assesing TLR and PAR2 crosstalk in epithelial cells during COPD exacerbations

Identifier:  TFG:6567

Handle: https://hdl.handle.net/20.500.11797/TFG6567

Authors:  Navarro Esclapés, Irene

Abstract:
Chronic obstructive pulmonary disease (COPD) is characterised by progressive deterioration of the airways after exposure to pollutants and tobacco smoke. Epithelial cells (ECs) express toll-like receptors (TLR) and protease-activated receptors (PAR) both proven to be involved in COPD pathogenesis. Characterising the expression of these receptors in respiratory ECs and studying whether there is a crosstalk between them during exacerbations -episodes of symptom worsening- is essential for a complete understanding of COPD. By using immunofluorescence, we proved the expression of PAR2, TLR3 and TLR4 on A549 cells (EC line) and by performing Enzyme-Linked Immuno Sorbent Assay (ELISA) we assessed some aspects of TLR and PAR2 involvement on inflammation during exacerbations.