Role of the nuclear receptor RORγ1 in the differentiation and function of brown adipocytes

Identifier:  TFG:6587

Handle: https://hdl.handle.net/20.500.11797/TFG6587

Authors:  Angrill Navarro, Aleix

Abstract:
Retinoic acid-related orphan receptor gamma 1 (RORγ1) is drawing interest as a therapeutic target for the treatment of obesity and diabetes. Preliminary results showed that RORγ1 is highly expressed in brown adipose tissue (BAT) and increases during differentiation of immortalized brown adipocytes (iBA). To elucidate its role in BAT, we silenced RORγ1 expression using small interfering RNAs and inhibited RORγ1 activity by RORγ inverse agonists in iBA preadipocytes. Although we did not obtain firm results regarding morphological differentiation changes and brown fat markers expression, we validated iBA preadipocytes transfection.