Extracellular Vesicles in Gestational Diabetes Mellitus: Biochemical Characterization and Clinical Implications

Identifier:  TFG:9003

Handle: https://hdl.handle.net/20.500.11797/TFG9003

Authors:  Chaczko Korzeniowska, Aleksandra Anna

Abstract:
Small extracellular vesicles (sEVs) mediate inter-organ communication and are essential for fetal development and maternal health. Their profile is altered in gestational diabetes mellitus (GDM). This project aimed to define the sEV “fingerprint” in GDM. sEVs and microbiota-derived sEVs (msEVs) were isolated from plasma of normal glucose tolerance women and GDM pregnancies using size exclusion chromatography and density gradient ultracentrifugation. Nanoparticle tracking and proteomic analyses were performed. An increased concentration of circulating sEVs and placenta-derived sEVs in umbilical cord blood was observed in both groups, with no change in msEVs. These findings suggest sEV alterations reflect placental responses to GDM