Paper-based potentiometric sensor for monitoring galactose levels

Entity: Universitat Rovira i Virgili (URV)

Confidenciality: No

Education area(s): Nanociència, Materials i Processos - Tecnologia Química de Frontera 

Title in different languages: Paper-based potentiometric sensor for monitoring galactose levels

Abstract: Paper-based potentiometric sensor for monitoring galactose levels Julio César Zuaznabar Gardona Nanoscience, Materials and Processes: Chemical Technology at the Frontier Master Programme 2015-2016 juliocesar.zuaznabar@estudiants.urv.cat Supervisor: Dr. Francisco J. Andrade Department of Analytical Chemistry and Organic Chemistry. Universitat Rovira i Virgili Campus Sescelades, c/ Marcel·lí Domingo, 1. Tarragona, 43007, Spain. Abstract Enzyme-based potentiometric sensors can become cost-effective platforms for the diagnosis of different diseases in a fast and reliable way. This work reports a paper-based potentiometric sensor as a new platform to evaluate galactose levels in blood samples for diagnosis and monitoring of galactosemia. The system works by detecting the hydrogen peroxide generated by the catalytic oxidation of galactose using the galactose oxidase enzyme immobilized on a substrate. Two approaches were employed to immobilize galactose oxidase, which is specific for galactose, on the surface of a piece of platinized paper: i) sandwiching it between two polymer layers and, ii) cross-linking it with an amine-rich polymer. An external oxidizing layer formed by MnO2 particles is used to eliminate redox interferences. The system shows, sensitivity values of 33 and 57 mV/decade for sandwiching and crosslinking respectively, a limit of detection of 0.5*10-3 mol/L and a linear range from 1*10-3 to 1*10-2 mol/L, well within the clinically relevant range. This novel biosensor allows to perform determinations of galactose in few minutes instead of the typical hours or days required by the current lab-based approach. Keywords: galactosemia, potentiometry, biosensor, galactose

Subject: Química

Academic year: 2015-2016

Language: Anglès

Work's public defense date: 2016-09-09

Subject areas: Chemistry

Student: Zuaznabar Gardona, Julio César

Department: Química Analítica i Química Orgànica

TFM credits: 24

Creation date in repository: 2017-01-13

Keywords: Potentiometry, biosensor, galactose

Title in original language: Paper-based potentiometric sensor for monitoring galactose levels

Project director: Andrade, Francisco Javier