Forensic Relevance of Microcystin Detection: A comparative Analysis of Cross-Reactivity Factors, Toxicity Equivalency Factors and Inhibition Equivalency Factors

Entity: Universitat Rovira i Virgili (URV)

Confidenciality: No

Education area(s): Genètica, Física i Química Forense

APS: No

Title in different languages: Forensic Relevance of Microcystin Detection: A comparative Analysis of Cross-Reactivity Factors, Toxicity Equivalency Factors and Inhibition Equivalency Factors

Abstract: Microcystins (MCs) and nodularin are cyanotoxins requiring accurate detection for effective risk management. This study investigates cross-reactivity factors (CRFs) using a colorimetric immunoassay with magnetic beads and reviews toxicity equivalency factors (TEFs), inhibition equivalency factors (IEFs), and CRFs from literature. Optimal assay conditions include 10 µg/mL MC-LR conjugated and 1:2500 monoclonal antibody dilution. Variants like MC-RR and nodularin showed good immunoreactivity, while MC-LY and MC-LW had poor antibody recognition. Variability between values is attributed to diverse experimental parameters and suggest an important role of pharmacokinetics.

Subject: Medicaments

Academic year: 2024-2025

Language: en

Work's public defense date: 2025-06-27

Subject areas: Health sciences

Student: Igual Cortiella, Maria

Work's codirector: Ronda Bargalló, Juan Carlos

Department: Química Analítica i Química Orgànica

Creation date in repository: 2025-11-27

Keywords: Microcystins, cyanotoxins, magnetic beads based immunoassay

Title in original language: Forensic Relevance of Microcystin Detection: A comparative Analysis of Cross-Reactivity Factors, Toxicity Equivalency Factors and Inhibition Equivalency Factors

Access Rights: info:eu-repo/semantics/openAccess

Project director: Campàs Homs, Mònica