Detection of glucose in potentiometric paper-based enzymatic biosensors using glucose dehydrogenase.

Entity: Universitat Rovira i Virgili (URV)

Confidenciality: No

Education area(s): Nanociència, Materials i Processos - Tecnologia Química de Frontera 

Title in different languages: Detection of glucose in potentiometric paper-based enzymatic biosensors using glucose dehydrogenase.

Abstract: A novel sensitive paper-based sensor for the potentiometric detection of glucose response is presented. Working and reference electrodes were built into a potentiometric cell in order to measure glucose levels through a new proposed reaction using co-enzymes and mediators. The working electrode consists on a platinum sputtered filter paper coated with a membrane of Nafion that entraps the glucose dehydrogenase enzyme with the NAD coenzyme. The reference electrode was developed by drop-casting of polyvinylbutyral-based membrane on a conductive paper coated with a layer of NaCl. Hydrogen peroxide was detected by the working electrode under influence enzymatic reaction. The response obtained was proportional to the logarithm of the concentration of glucose, with a sensitivity -95,9 ± 3,6 mV/decade, a linear range that spans from 0,3 mM to 10 mM and a limit of detection of 0,2 mM of glucose. This approach opens up the way for fabrication the new devices and prospects for diagnostic results.

Subject: Química

Academic year: 2016-2017

Language: Anglès

Work's public defense date: 2017-12-09

Subject areas: Chemistry

Student: Racka, Paulina

Work's codirector: Blondeau, Pascal

Department: Química Analítica i Química Orgànica

Creation date in repository: 2018-03-21

TFM credits: 24

Keywords: Glucose dehydrogenase enzyme, biosensors, potentiometry.

Title in original language: Detection of glucose in potentiometric paper-based enzymatic biosensors using glucose dehydrogenase.

Project director: Riu Rusell, Jordi