Detection of glucose in potentiometric paper-based enzymatic biosensors using glucose dehydrogenase.

Identifier:  TFM:301

Handle: https://hdl.handle.net/20.500.11797/TFM301

Authors:  Racka, Paulina

Abstract:
A novel sensitive paper-based sensor for the potentiometric detection of glucose response is presented. Working and reference electrodes were built into a potentiometric cell in order to measure glucose levels through a new proposed reaction using co-enzymes and mediators. The working electrode consists on a platinum sputtered filter paper coated with a membrane of Nafion that entraps the glucose dehydrogenase enzyme with the NAD coenzyme. The reference electrode was developed by drop-casting of polyvinylbutyral-based membrane on a conductive paper coated with a layer of NaCl. Hydrogen peroxide was detected by the working electrode under influence enzymatic reaction. The response obtained was proportional to the logarithm of the concentration of glucose, with a sensitivity -95,9 ± 3,6 mV/decade, a linear range that spans from 0,3 mM to 10 mM and a limit of detection of 0,2 mM of glucose. This approach opens up the way for fabrication the new devices and prospects for diagnostic results.