Direct electrical contacting of pyrroloquinoline quinone dependent glucose dehydrogenase (PQQ-GDH) based on polyaniline and carbon nano onion-modified gold electrodes

Entity: Universitat Rovira i Virgili (URV)

Confidenciality: No

Education area(s): Nanociència, Materials i Processos - Tecnologia Química de Frontera 

Title in different languages: Direct electrical contacting of pyrroloquinoline quinone dependent glucose dehydrogenase (PQQ-GDH) based on polyaniline and carbon nano onion-modified gold electrodes

Abstract: The interaction of enzymes with electrodes is critical for the integration of bioelectronic systems for applications in biosensors and bionic devices for energy harvesting or biocomputing. To create such devices and systems that are robust, direct electron transfer (DET) between enzyme and electrode is the deciding factor for sensitivity and reliability. This work focuses on fabricating such a reliable bioelectronic interface based on pyrroloquinoline quinone-dependent glucose dehydrogenase [PQQ-GDH, EC 1.1.5.2]. In order to achieve DET, the electrode surface was modified by carbon nano onions (CNO) and polyaniline copolymer on gold electrodes that facilitated direct electron transfer in the presence of glucose. The CNO were adsorbed on to the surface and polymer film was formed by co-electropolymerization of 2-amino benzoic acid (ABA) and 3-aminophenyl boronic acid (APBA) monomers. The resulting electrodes show behaviour that can be interpreted as direct electron transfer. At the enzyme saturation, the current density of 120 μA/cm2 was achieved. This indicates that the CNO modified electrodes are capable of glucose electro-oxidation and polymer acts as conductive film improving the electron transfer. Preliminary attempts to optimise the electrode modification conditions and the effect of operating variables are reported.

Subject: Bioquímica i biotecnologia

Academic year: 2019-2020

Language: Anglès

Work's public defense date: 2020-09-09

Subject areas: Biochemistry and biotechnology

Student: Chandra Pandurangi, Naga Adithya

Department: Enginyeria Química

Creation date in repository: 2021-07-30

TFM credits: 24

Keywords: PQQ-GDH, Direct electron transfer, carbon nano onions

Title in original language: Direct electrical contacting of pyrroloquinoline quinone dependent glucose dehydrogenase (PQQ-GDH) based on polyaniline and carbon nano onion-modified gold electrodes

Access Rights: info:eu-repo/semantics/openAccess

Project director: Katakis, Ioanis