Articles producció científica> Bioquímica i Biotecnologia

Determination of dehydrogenase activities involved in D-glucose oxidation in Gluconobacter and Acetobacter strains

  • Dades identificatives

    Identificador: PC:1920
    Handle: https://hdl.handle.net/20.500.11797/PC1920
    Autors:
    María Jesús TorijaFlorencia SainzMinenosuke MatsutaniNaoya KataokaToshiharu YakushiKazunobu MatsushitaAlbert Mas
    Resum:
    Acetic acid bacteria (AAB) are known for rapid and incomplete oxidation of an extensively variety of alcohols and carbohydrates, resulting in the accumulation of organic acids as the final products. These oxidative fermentations in AAB are catalyzed by PQQ- or FAD- dependent membrane-bound dehydrogenases. In the present study, the enzyme activity of the membrane-bound dehydrogenases [membrane-bound PQQ-glucose dehydrogenase (mGDH), D-gluconate dehydrogenase (GADH) and membrane-bound glycerol dehydrogenase (GLDH)] involved in the oxidation of D-glucose and D-gluconic acid (GA) was determined in six strains of three different species of AAB (three natural and three type strains). Moreover, the effect of these activities on the production of related metabolites [GA, 2-keto-D-gluconic acid (2KGA) and 5-keto-D-gluconic acid (5KGA)] was analyzed. The natural strains belonging to Gluconobacter showed a high mGDH activity and low activity in GADH and GLDH, whereas the Acetobacter malorum strain presented low activity in the three enzymes. Nevertheless, no correlation was observed between the activity of these enzymes and the concentration of the corresponding metabolites. In fact, all the tested strains were able to oxidize D-glucose to GA, being maximal at the late exponential phase of the AAB growth (24 h), which coincided with D-glucose exhaustion and the maximum mGDH activity. Instead, only some of the tested strains were capable of producing 2KGA and/or 5KGA. In the case of Gluconobacter oxydans strains, no 2KGA production was detected which is related to the absence of GADH activity after 24 h, while in the remaining strains, detection of GADH activity after 24 h resulted in a high accumulation of 2KGA. Therefore, it is possible to choose the best strain depending on the

  • Altres:

    Autor segons l'article: María Jesús Torija; Florencia Sainz; Minenosuke Matsutani; Naoya Kataoka; Toshiharu Yakushi; Kazunobu Matsushita; Albert Mas
    Departament: Bioquímica i Biotecnologia
    Autor/s de la URV: TORIJA MARTÍNEZ, MARÍA JESÚS; Florencia Sainz; Minenosuke Matsutani; Naoya Kataoka; Toshiharu Yakushi; Kazunobu Matsushita; MAS BARON, ALBERTO
    Paraules clau: Keto-D-gluconic acids D-gluconic acid Acetic acid bacteria
    Resum: Acetic acid bacteria (AAB) are known for rapid and incomplete oxidation of an extensively variety of alcohols and carbohydrates, resulting in the accumulation of organic acids as the final products. These oxidative fermentations in AAB are catalyzed by PQQ- or FAD- dependent membrane-bound dehydrogenases. In the present study, the enzyme activity of the membrane-bound dehydrogenases [membrane-bound PQQ-glucose dehydrogenase (mGDH), D-gluconate dehydrogenase (GADH) and membrane-bound glycerol dehydrogenase (GLDH)] involved in the oxidation of D-glucose and D-gluconic acid (GA) was determined in six strains of three different species of AAB (three natural and three type strains). Moreover, the effect of these activities on the production of related metabolites [GA, 2-keto-D-gluconic acid (2KGA) and 5-keto-D-gluconic acid (5KGA)] was analyzed. The natural strains belonging to Gluconobacter showed a high mGDH activity and low activity in GADH and GLDH, whereas the Acetobacter malorum strain presented low activity in the three enzymes. Nevertheless, no correlation was observed between the activity of these enzymes and the concentration of the corresponding metabolites. In fact, all the tested strains were able to oxidize D-glucose to GA, being maximal at the late exponential phase of the AAB growth (24 h), which coincided with D-glucose exhaustion and the maximum mGDH activity. Instead, only some of the tested strains were capable of producing 2KGA and/or 5KGA. In the case of Gluconobacter oxydans strains, no 2KGA production was detected which is related to the absence of GADH activity after 24 h, while in the remaining strains, detection of GADH activity after 24 h resulted in a high accumulation of 2KGA. Therefore, it is possible to choose the best strain depending on the desired product composition. Moreover, the sequences of these genes were used to construct phylogenetic trees. According to the sequence of gcd, gene coding for mGDH, Acetobacter and Komagataeibacter were phylogenetically more closely related each other than with Gluconobacter.
    Grup de recerca: Biotecnologia Enològica
    Àrees temàtiques: Bioquímica i biotecnologia Bioquímica y tecnología Biochemistry and technology
    Accès a la llicència d'ús: https://creativecommons.org/licenses/by/3.0/es/
    ISSN: 1664-302X
    Identificador de l'autor: 0000-0001-6419-0745; n/a; n/a; n/a; n/a; n/a; 0000-0002-0763-1679
    Data d'alta del registre: 2016-10-17
    Volum de revista: 7
    Versió de l'article dipositat: info:eu-repo/semantics/publishedVersion
    Enllaç font original: https://www.frontiersin.org/articles/10.3389/fmicb.2016.01358/full
    URL Document de llicència: https://repositori.urv.cat/ca/proteccio-de-dades/
    DOI de l'article: 10.3389/fmicb.2016.01358
    Entitat: Universitat Rovira i Virgili
    Any de publicació de la revista: 2016
    Pàgina inicial: Art.num. 1358
    Tipus de publicació: Article Artículo Article

  • Paraules clau:

    Acetobàcter
    Keto-D-gluconic acids
    D-gluconic acid
    Acetic acid bacteria
    Bioquímica i biotecnologia
    Bioquímica y tecnología
    Biochemistry and technology
    1664-302X

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