Autor segons l'article: Fernández-Castillejo, Sara; Macià, Alba; Motilva, Maria-José; Catalán, Úrsula; Solà, Rosa
Departament: Medicina i Cirurgia
Autor/s de la URV: FERNÁNDEZ CASTILLEJO, SARA; Macià, Alba; Motilva, Maria-José; CATALÁN SANTOS, ÚRSULA; SOLÀ ALBERICH, ROSA MARIA
Paraules clau: resveratrol piceid Metabolisme
Resum: Scope. The antithrombotic effect of resveratrol (RV) and its forms remains unknown. The objective is to evaluate the effect of RV, its glucoside form piceid, and its biological metabolites (RV-3-O-β-D-Glucuronide, RV-4’-O-D-Glucuronide, and RV-3-O-Sulfate) on Tissue Factor (TF) modulation. Moreover, the endothelial metabolism of RV is assessed. Methods and results. Human aortic endothelial cells were incubated with trans-piceid, trans-RV, or its biological metabolites and stimulated with TNF-α. TF activity, protein, and mRNA expression were determined in cell lysates. Moreover, RV conjugation (phase II metabolism) to its sulphated or glucuronidated metabolites and their deconjugation to their parent compound (free RV) were also assessed in cell lysates and culture media. RV decreased TF activity, protein, and mRNA expression whereas piceid and RV metabolites showed no effects. RV-3-O-Sulfate was the main metabolite generated in the endothelium, while RV metabolites were deconjugated to free RV. Trans-RV and its trans-metabolites isomerization was observed generating their cis-forms. Conclusions. RV boasts antithrombotic effects modulating TF. RV metabolites and piceid did not show this effect. However, endothelial cells capacity to deconjugate RV metabolites to free RV indicates that RV metabolites can act as an endothelial reservoir for RV regeneration contributing to the antithrombotic effects of RV.
Grup de recerca: Unitat de Recerca de Lípids i Arteriosclerosi
Àrees temàtiques: Ciències de la salut Ciencias de la salud Health sciences
Accès a la llicència d'ús: https://creativecommons.org/licenses/by/3.0/es/
ISSN: 1613-4125
Identificador de l'autor: 0000-0002-8109-9713; 0000-0001-6091-5606; 0000-0003-3823-3953; 0000-0001-8884-9823; 0000-0002-8359-235X
Data d'alta del registre: 2019-03-21
Volum de revista: 63
Versió de l'article dipositat: info:eu-repo/semantics/submittedVersion
Enllaç font original: https://onlinelibrary.wiley.com/doi/10.1002/mnfr.201800715
Programa de finançament: plan; AppleCOR; AGL2016-76943-C2-2-R altres; CENIT; MET-DEV-FUN
URL Document de llicència: https://repositori.urv.cat/ca/proteccio-de-dades/
DOI de l'article: 10.1002/mnfr.201800715
Entitat: Universitat Rovira i Virgili
Any de publicació de la revista: 2018
Pàgina inicial: 1800715
Tipus de publicació: Article Artículo Article
Repositori URV