Author, as appears in the article.: Fernández-Castillejo, Sara; Macià, Alba; Motilva, Maria-José; Catalán, Úrsula; Solà, Rosa
Department: Medicina i Cirurgia
URV's Author/s: FERNÁNDEZ CASTILLEJO, SARA; Macià, Alba; Motilva, Maria-José; CATALÁN SANTOS, ÚRSULA; SOLÀ ALBERICH, ROSA MARIA
Keywords: resveratrol piceid Metabolisme
Abstract: Scope. The antithrombotic effect of resveratrol (RV) and its forms remains unknown. The objective is to evaluate the effect of RV, its glucoside form piceid, and its biological metabolites (RV-3-O-β-D-Glucuronide, RV-4’-O-D-Glucuronide, and RV-3-O-Sulfate) on Tissue Factor (TF) modulation. Moreover, the endothelial metabolism of RV is assessed. Methods and results. Human aortic endothelial cells were incubated with trans-piceid, trans-RV, or its biological metabolites and stimulated with TNF-α. TF activity, protein, and mRNA expression were determined in cell lysates. Moreover, RV conjugation (phase II metabolism) to its sulphated or glucuronidated metabolites and their deconjugation to their parent compound (free RV) were also assessed in cell lysates and culture media. RV decreased TF activity, protein, and mRNA expression whereas piceid and RV metabolites showed no effects. RV-3-O-Sulfate was the main metabolite generated in the endothelium, while RV metabolites were deconjugated to free RV. Trans-RV and its trans-metabolites isomerization was observed generating their cis-forms. Conclusions. RV boasts antithrombotic effects modulating TF. RV metabolites and piceid did not show this effect. However, endothelial cells capacity to deconjugate RV metabolites to free RV indicates that RV metabolites can act as an endothelial reservoir for RV regeneration contributing to the antithrombotic effects of RV.
Research group: Unitat de Recerca de Lípids i Arteriosclerosi
Thematic Areas: Ciències de la salut Ciencias de la salud Health sciences
licence for use: https://creativecommons.org/licenses/by/3.0/es/
ISSN: 1613-4125
Author identifier: 0000-0002-8109-9713; 0000-0001-6091-5606; 0000-0003-3823-3953; 0000-0001-8884-9823; 0000-0002-8359-235X
Record's date: 2019-03-21
Journal volume: 63
Papper version: info:eu-repo/semantics/submittedVersion
Link to the original source: https://onlinelibrary.wiley.com/doi/10.1002/mnfr.201800715
Funding program: plan; AppleCOR; AGL2016-76943-C2-2-R altres; CENIT; MET-DEV-FUN
Licence document URL: https://repositori.urv.cat/ca/proteccio-de-dades/
Article's DOI: 10.1002/mnfr.201800715
Entity: Universitat Rovira i Virgili
Journal publication year: 2018
First page: 1800715
Publication Type: Article Artículo Article
Repositori URV