Autor segons l'article: Sembongi, H. Miranda, M. Han, G.-S. Fakas, S. Grimsey, N. Vendrell, J. Carman, G.M. Siniossoglou, S.
Versió de l'article dipositat: info:eu-repo/semantics/publishedVersion
Departament: Medicina i Cirurgia
e-ISSN: 1083-351X
URL Document de llicència: https://repositori.urv.cat/ca/proteccio-de-dades/
Resum: Lipins are evolutionarily conserved Mg2+-dependent phosphatidate phosphatase (PAP) enzymes with essential roles in lipid biosynthesis. Mammals express three paralogues: lipins 1, 2, and 3. Loss of lipin 1 in mice inhibits adipogenesis at an early stage of differentiation and results in a lipodystrophic phenotype. The role of lipins at later stages of adipogenesis, when cells initiate the formation of lipid droplets, is less well characterized. We found that depletion of lipin 1, after the initiation of differentiation in 3T3-L1 cells but before the loading of lipid droplets with triacylglycerol, results in a reciprocal increase of lipin 2, but not lipin 3. We generated 3T3-L1 cells where total lipin protein and PAP activity levels are down-regulated by the combined depletion of lipins 1 and 2 at day 4 of differentiation. These cells still accumulated triacylglycerol but displayed a striking fragmentation of lipid droplets without significantly affecting their total volume per cell. This was due to the lack of the PAP activity of lipin 1 in adipocytes after day 4 of differentiation, whereas depletion of lipin 2 led to an increase of lipid droplet volume per cell. We propose that in addition to their roles during early adipogenesis, lipins also have a role in lipid droplet biogenesis.
Entitat: Universitat Rovira i Virgili.
Any de publicació de la revista: 2013
Accès a la llicència d'ús: https://creativecommons.org/licenses/by/3.0/es/
Pàgina inicial: 34502
Pàgina final: 34513
Volum de revista: 288
Repositori URV