Autor según el artículo: Sembongi, H. Miranda, M. Han, G.-S. Fakas, S. Grimsey, N. Vendrell, J. Carman, G.M. Siniossoglou, S.
Departamento: Medicina i Cirurgia
e-ISSN: 1083-351X
Resumen: Lipins are evolutionarily conserved Mg2+-dependent phosphatidate phosphatase (PAP) enzymes with essential roles in lipid biosynthesis. Mammals express three paralogues: lipins 1, 2, and 3. Loss of lipin 1 in mice inhibits adipogenesis at an early stage of differentiation and results in a lipodystrophic phenotype. The role of lipins at later stages of adipogenesis, when cells initiate the formation of lipid droplets, is less well characterized. We found that depletion of lipin 1, after the initiation of differentiation in 3T3-L1 cells but before the loading of lipid droplets with triacylglycerol, results in a reciprocal increase of lipin 2, but not lipin 3. We generated 3T3-L1 cells where total lipin protein and PAP activity levels are down-regulated by the combined depletion of lipins 1 and 2 at day 4 of differentiation. These cells still accumulated triacylglycerol but displayed a striking fragmentation of lipid droplets without significantly affecting their total volume per cell. This was due to the lack of the PAP activity of lipin 1 in adipocytes after day 4 of differentiation, whereas depletion of lipin 2 led to an increase of lipid droplet volume per cell. We propose that in addition to their roles during early adipogenesis, lipins also have a role in lipid droplet biogenesis.
Acceso a la licencia de uso: https://creativecommons.org/licenses/by/3.0/es/
Página final: 34513
Volumen de revista: 288
Versión del articulo depositado: info:eu-repo/semantics/publishedVersion
Enlace a la fuente original: http://www.jbc.org/content/288/48/34502
URL Documento de licencia: https://repositori.urv.cat/ca/proteccio-de-dades/
DOI del artículo: 10.1074/jbc.M113.488445
Entidad: Universitat Rovira i Virgili.
Año de publicación de la revista: 2013
Página inicial: 34502
Repositori URV