Autor segons l'article: Talagrand-Reboul E; Latif-Eugenín F; Beaz-Hidalgo R; Colston S; Figueras M; Graf J; Jumas-Bilak E; Lamy B
Departament: Ciències Mèdiques Bàsiques
Autor/s de la URV: BEAZ HIDALGO, ROXANA / Figueras Salvat, María Josefa
Paraules clau: Good health and well-being
Resum: Copyright © 2018 Talagrand-Reboul et al. Many virulence factors have been described for opportunistic pathogens within the genus Aeromonas. Polymerase Chain Reactions (PCRs) are commonly used in population studies of aeromonads to detect virulence-associated genes in order to better understand the epidemiology and emergence of Aeromonas from the environment to host, but their performances have never been thoroughly evaluated. We aimed to determine diagnostic sensitivity and specificity of PCR assays for the detection of virulence-associated genes in a collection of Aeromonas isolates representative for the genetic diversity in the genus. Thirty-nine Aeromonas strains belonging to 27 recognized species were screened by published PCR assays for virulence-associated genes (act, aerA, aexT, alt, ascFG, ascV, ast, lafA, lip, ser, stx1, stx2A). In parallel, homologues of the 12 putative virulence genes were searched from the genomes of the 39 strains. Of the 12 published PCR assays for virulence factors, the comparison of PCR results and genome analysis estimated diagnostic sensitivities ranging from 34% to 100% and diagnostic specificities ranged from 71% to 100% depending upon the gene. To improve the detection of virulence-associated genes in aeromonads, we have designed new primer pairs for aerA/act, ser, lafA, ascFG and ascV, which showed excellent diagnostic sensitivity and specificity. Altogether, the analysis of high quality genomic data, which are more and more easy to obtain, provides significant improvements in the genetic detection of virulence factors in bacterial strains.
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Accès a la llicència d'ús: https://creativecommons.org/licenses/by/3.0/es/
ISSN: 19326203
Adreça de correu electrònic de l'autor: mariajose.figueras@urv.cat
Identificador de l'autor: 0000-0002-2268-8980
Data d'alta del registre: 2024-11-16
Versió de l'article dipositat: info:eu-repo/semantics/publishedVersion
Enllaç font original: https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0201428
URL Document de llicència: https://repositori.urv.cat/ca/proteccio-de-dades/
Referència a l'article segons font original: Plos One. 13 (8): e0201428-e0201428
Referència de l'ítem segons les normes APA: Talagrand-Reboul E; Latif-Eugenín F; Beaz-Hidalgo R; Colston S; Figueras M; Graf J; Jumas-Bilak E; Lamy B (2018). Genome-driven evaluation and redesign of PCR tools for improving the detection of virulence-associated genes in aeromonads. Plos One, 13(8), e0201428-e0201428. DOI: 10.1371/journal.pone.0201428
DOI de l'article: 10.1371/journal.pone.0201428
Entitat: Universitat Rovira i Virgili
Any de publicació de la revista: 2018
Tipus de publicació: Journal Publications