Autor según el artículo: Talagrand-Reboul E; Latif-Eugenín F; Beaz-Hidalgo R; Colston S; Figueras M; Graf J; Jumas-Bilak E; Lamy B
Departamento: Ciències Mèdiques Bàsiques
Autor/es de la URV: BEAZ HIDALGO, ROXANA / Figueras Salvat, María Josefa
Palabras clave: @infoAeu @residentesaeu @uroweb Etiqueta «#» Hashtag
Resumen: Copyright © 2018 Talagrand-Reboul et al. Many virulence factors have been described for opportunistic pathogens within the genus Aeromonas. Polymerase Chain Reactions (PCRs) are commonly used in population studies of aeromonads to detect virulence-associated genes in order to better understand the epidemiology and emergence of Aeromonas from the environment to host, but their performances have never been thoroughly evaluated. We aimed to determine diagnostic sensitivity and specificity of PCR assays for the detection of virulence-associated genes in a collection of Aeromonas isolates representative for the genetic diversity in the genus. Thirty-nine Aeromonas strains belonging to 27 recognized species were screened by published PCR assays for virulence-associated genes (act, aerA, aexT, alt, ascFG, ascV, ast, lafA, lip, ser, stx1, stx2A). In parallel, homologues of the 12 putative virulence genes were searched from the genomes of the 39 strains. Of the 12 published PCR assays for virulence factors, the comparison of PCR results and genome analysis estimated diagnostic sensitivities ranging from 34% to 100% and diagnostic specificities ranged from 71% to 100% depending upon the gene. To improve the detection of virulence-associated genes in aeromonads, we have designed new primer pairs for aerA/act, ser, lafA, ascFG and ascV, which showed excellent diagnostic sensitivity and specificity. Altogether, the analysis of high quality genomic data, which are more and more easy to obtain, provides significant improvements in the genetic detection of virulence factors in bacterial strains.
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Acceso a la licencia de uso: https://creativecommons.org/licenses/by/3.0/es/
Direcció de correo del autor: mariajose.figueras@urv.cat
ISSN: 19326203
Identificador del autor: 0000-0002-2268-8980
Fecha de alta del registro: 2023-02-22
Versión del articulo depositado: info:eu-repo/semantics/publishedVersion
Enlace a la fuente original: https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0201428
Referencia al articulo segun fuente origial: Plos One. 13 (8): e0201428-
Referencia de l'ítem segons les normes APA: Talagrand-Reboul E; Latif-Eugenín F; Beaz-Hidalgo R; Colston S; Figueras M; Graf J; Jumas-Bilak E; Lamy B (2018). Genome-driven evaluation and redesign of PCR tools for improving the detection of virulence-associated genes in aeromonads. Plos One, 13(8), e0201428-. DOI: 10.1371/journal.pone.0201428
URL Documento de licencia: http://repositori.urv.cat/ca/proteccio-de-dades/
DOI del artículo: 10.1371/journal.pone.0201428
Entidad: Universitat Rovira i Virgili
Año de publicación de la revista: 2018
Tipo de publicación: Journal Publications