Articles producció científica> Enginyeria Química

In-vitro metabolomics to evaluate toxicity of particulate matter under environmentally realistic conditions

  • Dades identificatives

    Identificador: imarina:4683849
    Autors:
    Sanchez-Soberon, FranciscoCuykx, MatthiasSerra, NoemiLinares, VictoriaBelles, MontserratCovaci, AdrianSchuhmacher, Marta
    Resum:
    In this pilot study three fractions of particulate matter (PM0.25, PM2.5-0.25, and PM10-2.5) were collected in three environments (classroom, home, and outdoors) in a village located nearby an industrial complex. Time-activity pattern of 20 students attending the classroom was obtained, and the dose of particles reaching the children's lungs under actual environmental conditions (i.e. real dose) was calculated via dosimetry model. The highest PM concentrations were reached in the classroom. Simulations showed that heavy intensity outdoor activities played a major role in PM deposition, especially in the upper part of the respiratory tract. The mass of PM10-2.5 reaching the alveoli was minor, while PM2.5-0.25 and PM0.25 apportion for most of the PM mass retained in the lungs. Consequently, PM2.5-0.25 and PM0.25 were the only fractions used in two subsequent toxicity assays onto alveolar cells (A549). First, a cytotoxicity dose-response assay was performed, and doses corresponding to 5% mortality (LC5) were estimated. Afterwards, two LC-MS metabolomic assays were conducted: one applying LC5, and another applying real dose. A lower estimated LC5 value was obtained for PM0.25 than PM2.5-0.25 (8.08 and 73.7 ng/mL respectively). The number of altered features after LC5 exposure was similar for both fractions (39 and 38 for PM0.25 and PM2.5-0.25 respectively), while after real dose exposure these numbers differed (10 and 5 for PM0.25 and PM2.5-0.25 respectively). The most metabolic changes were related to membrane and lung surfactant lipids. This study highlights the capacity of PM to alter metabolic profile of lung cells at conventional environmental levels.
  • Altres:

    Autor segons l'article: Sanchez-Soberon, Francisco; Cuykx, Matthias; Serra, Noemi; Linares, Victoria; Belles, Montserrat; Covaci, Adrian; Schuhmacher, Marta
    Departament: Ciències Mèdiques Bàsiques Enginyeria Química
    Autor/s de la URV: Bellés Mateu, Montserrat / Linares Vidal, M. Victoria / SÁNCHEZ SOBERÓN, FRANCISCO / Schuhmacher Ansuategui, Marta
    Paraules clau: Particulate matter Metabolomics Indoor sampling Hp-lc/ms Cytotoxicity A549 metabolomics indoor sampling hp-lc/ms cytotoxicity a549
    Resum: In this pilot study three fractions of particulate matter (PM0.25, PM2.5-0.25, and PM10-2.5) were collected in three environments (classroom, home, and outdoors) in a village located nearby an industrial complex. Time-activity pattern of 20 students attending the classroom was obtained, and the dose of particles reaching the children's lungs under actual environmental conditions (i.e. real dose) was calculated via dosimetry model. The highest PM concentrations were reached in the classroom. Simulations showed that heavy intensity outdoor activities played a major role in PM deposition, especially in the upper part of the respiratory tract. The mass of PM10-2.5 reaching the alveoli was minor, while PM2.5-0.25 and PM0.25 apportion for most of the PM mass retained in the lungs. Consequently, PM2.5-0.25 and PM0.25 were the only fractions used in two subsequent toxicity assays onto alveolar cells (A549). First, a cytotoxicity dose-response assay was performed, and doses corresponding to 5% mortality (LC5) were estimated. Afterwards, two LC-MS metabolomic assays were conducted: one applying LC5, and another applying real dose. A lower estimated LC5 value was obtained for PM0.25 than PM2.5-0.25 (8.08 and 73.7 ng/mL respectively). The number of altered features after LC5 exposure was similar for both fractions (39 and 38 for PM0.25 and PM2.5-0.25 respectively), while after real dose exposure these numbers differed (10 and 5 for PM0.25 and PM2.5-0.25 respectively). The most metabolic changes were related to membrane and lung surfactant lipids. This study highlights the capacity of PM to alter metabolic profile of lung cells at conventional environmental levels.
    Àrees temàtiques: Zootecnia / recursos pesqueiros Saúde coletiva Química Public health, environmental and occupational health Pollution Odontología Nutrição Medicine (miscellaneous) Medicina veterinaria Medicina ii Medicina i Materiais Interdisciplinar Health, toxicology and mutagenesis Geociências General chemistry Farmacia Environmental sciences Environmental engineering Environmental chemistry Ensino Engenharias iii Engenharias ii Engenharias i Educação física Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Ciências ambientais Ciências agrárias i Ciência de alimentos Chemistry (miscellaneous) Chemistry (all) Biotecnología Biodiversidade Astronomia / física
    Accès a la llicència d'ús: https://creativecommons.org/licenses/by/3.0/es/
    ISSN: 00456535
    Adreça de correu electrònic de l'autor: mvictoria.linares@urv.cat montserrat.belles@urv.cat marta.schuhmacher@urv.cat
    Identificador de l'autor: 0000-0003-4170-4197 0000-0002-9282-2989 0000-0003-4381-2490
    Data de publicació de l'article: 2018/10/31
    Data d'alta del registre: 2024-10-12
    Versió de l'article dipositat: info:eu-repo/semantics/acceptedVersion
    Enllaç font original: https://www.sciencedirect.com/science/article/abs/pii/S0045653518311408?via%3Dihub
    URL Document de llicència: https://repositori.urv.cat/ca/proteccio-de-dades/
    Referència a l'article segons font original: Chemosphere. 209 137-146
    Referència de l'ítem segons les normes APA: Sanchez-Soberon, Francisco; Cuykx, Matthias; Serra, Noemi; Linares, Victoria; Belles, Montserrat; Covaci, Adrian; Schuhmacher, Marta (2018). In-vitro metabolomics to evaluate toxicity of particulate matter under environmentally realistic conditions. Chemosphere, 209(), 137-146. DOI: 10.1016/j.chemosphere.2018.06.065
    DOI de l'article: 10.1016/j.chemosphere.2018.06.065
    Entitat: Universitat Rovira i Virgili
    Any de publicació de la revista: 2018
    Tipus de publicació: Journal Publications
  • Paraules clau:

    Chemistry (Miscellaneous),Environmental Chemistry,Environmental Engineering,Environmental Sciences,Health, Toxicology and Mutagenesis,Medicine (Miscellaneous),Pollution,Public Health, Environmental and Occupational Health
    Particulate matter
    Metabolomics
    Indoor sampling
    Hp-lc/ms
    Cytotoxicity
    A549
    metabolomics
    indoor sampling
    hp-lc/ms
    cytotoxicity
    a549
    Zootecnia / recursos pesqueiros
    Saúde coletiva
    Química
    Public health, environmental and occupational health
    Pollution
    Odontología
    Nutrição
    Medicine (miscellaneous)
    Medicina veterinaria
    Medicina ii
    Medicina i
    Materiais
    Interdisciplinar
    Health, toxicology and mutagenesis
    Geociências
    General chemistry
    Farmacia
    Environmental sciences
    Environmental engineering
    Environmental chemistry
    Ensino
    Engenharias iii
    Engenharias ii
    Engenharias i
    Educação física
    Ciências biológicas iii
    Ciências biológicas ii
    Ciências biológicas i
    Ciências ambientais
    Ciências agrárias i
    Ciência de alimentos
    Chemistry (miscellaneous)
    Chemistry (all)
    Biotecnología
    Biodiversidade
    Astronomia / física
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